TY - JOUR
T1 - Absence of a Classically Activated Macrophage Cytokine Signature in Peripheral Spondylarthritis, Including Psoriatic Arthritis
AU - Vandooren, Bernard
AU - Noordenbos, Troy
AU - Ambarus, Carmen
AU - Krausz, Sarah
AU - Cantaert, Tineke
AU - Yeremenko, Nataliya
AU - Boumans, Maartje
AU - Lutter, Rene
AU - Tak, Paul P.
AU - Baeten, Dominique
PY - 2009
Y1 - 2009
N2 - Objective. Peripheral spondylarthritis (SpA) is characterized by macrophages that express CD163, a marker of alternative activation (M2). The purpose of this study was to assess whether this differential infiltration with macrophage subsets was associated with a different local inflammatory milieu in SpA as compared with rheumatoid arthritis (RA). Methods. The effect of SpA and RA synovial fluid (SF) on macrophage polarization was tested in vitro on normal peripheral blood monocytes. SF levels of classically activated macrophage (M1)-derived and alternatively activated macrophage (M2)-derived mediators were analyzed by enzyme-linked immunosorbent assay and multiparameter Luminex bead assay in 47 patients with non-psoriatic SpA, 55 with RA, and 15 with psoriatic arthritis (PsA). Paired synovial biopsy samples were analyzed histologically. Results. SF from SpA patients promoted preferential expression of the M2 markers CD163 and CD200R in vitro, even if SF levels of the prototypical M2-polarizing factors (interleukin-4 [IL-4], IL-13, and IL-10) were not increased as compared with those in RA SF. Despite a similar degree of overall joint inflammation in SpA and RA, SpA synovitis displayed strongly reduced SF levels of MI-derived, but not M2-derived, mediators, such as tumor necrosis factor alpha (TNF alpha), IL-1 beta, IL-12p70, and interferon-gamma-inducible protein 10. SF levels of MI-derived mediators correlated well with peripheral joint inflammation in RA, but neither these mediators nor IL-1 alpha and IL-17 did so in SpA. Of interest, the SF cytokine profile in PsA, a more destructive subtype of SpA, was similar to that in non-psoriatic SpA. Conclusion. The local inflammatory milieu is clearly different in SpA as compared with RA peripheral arthritis. Synovitis in SpA, including that in PsA, is characterized by a selective decrease in M1-derived proinflammatory mediators, such as TNF alpha and IL-1 beta
AB - Objective. Peripheral spondylarthritis (SpA) is characterized by macrophages that express CD163, a marker of alternative activation (M2). The purpose of this study was to assess whether this differential infiltration with macrophage subsets was associated with a different local inflammatory milieu in SpA as compared with rheumatoid arthritis (RA). Methods. The effect of SpA and RA synovial fluid (SF) on macrophage polarization was tested in vitro on normal peripheral blood monocytes. SF levels of classically activated macrophage (M1)-derived and alternatively activated macrophage (M2)-derived mediators were analyzed by enzyme-linked immunosorbent assay and multiparameter Luminex bead assay in 47 patients with non-psoriatic SpA, 55 with RA, and 15 with psoriatic arthritis (PsA). Paired synovial biopsy samples were analyzed histologically. Results. SF from SpA patients promoted preferential expression of the M2 markers CD163 and CD200R in vitro, even if SF levels of the prototypical M2-polarizing factors (interleukin-4 [IL-4], IL-13, and IL-10) were not increased as compared with those in RA SF. Despite a similar degree of overall joint inflammation in SpA and RA, SpA synovitis displayed strongly reduced SF levels of MI-derived, but not M2-derived, mediators, such as tumor necrosis factor alpha (TNF alpha), IL-1 beta, IL-12p70, and interferon-gamma-inducible protein 10. SF levels of MI-derived mediators correlated well with peripheral joint inflammation in RA, but neither these mediators nor IL-1 alpha and IL-17 did so in SpA. Of interest, the SF cytokine profile in PsA, a more destructive subtype of SpA, was similar to that in non-psoriatic SpA. Conclusion. The local inflammatory milieu is clearly different in SpA as compared with RA peripheral arthritis. Synovitis in SpA, including that in PsA, is characterized by a selective decrease in M1-derived proinflammatory mediators, such as TNF alpha and IL-1 beta
U2 - https://doi.org/10.1002/art.24406
DO - https://doi.org/10.1002/art.24406
M3 - Article
C2 - 19333931
SN - 0004-3591
VL - 60
SP - 966
EP - 975
JO - Arthritis and rheumatism
JF - Arthritis and rheumatism
IS - 4
ER -