TY - JOUR
T1 - Acquisition of high quality DNA for massive parallel sequencing by in vivo chromatin immunoprecipitation
AU - van den Boogaard, M.
AU - Wong, L. Y. E.
AU - Christoffels, V. M.
AU - Barnett, P.
PY - 2013
Y1 - 2013
N2 - ChIP-seq is rapidly becoming a routine technique for the determination of the genome wide association of DNA binding proteins and histone modifications. Here we provide a protocol for the isolation, purification, and immunoprecipitation of DNA fragments associated with a target transcription factor of interest. Although the method makes use of adult mouse hearts, it can, with relative ease, be adapted for the in vivo ChIP isolation of DNA from other cell and tissue sources with the intention of massive parallel sequencing
AB - ChIP-seq is rapidly becoming a routine technique for the determination of the genome wide association of DNA binding proteins and histone modifications. Here we provide a protocol for the isolation, purification, and immunoprecipitation of DNA fragments associated with a target transcription factor of interest. Although the method makes use of adult mouse hearts, it can, with relative ease, be adapted for the in vivo ChIP isolation of DNA from other cell and tissue sources with the intention of massive parallel sequencing
U2 - https://doi.org/10.1007/978-1-62703-284-1_5
DO - https://doi.org/10.1007/978-1-62703-284-1_5
M3 - Article
C2 - 23436353
SN - 1064-3745
VL - 977
SP - 53
EP - 64
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -