TY - JOUR
T1 - Adenoviral overexpression of apolipoprotein A-V reduces serum levels of triglycerides and cholesterol in mice
AU - van der Vliet, Hendrik N.
AU - Schaap, Frank G.
AU - Levels, Johannes H. M.
AU - Ottenhoff, Roelof
AU - Looije, Norbert
AU - Wesseling, John G.
AU - Groen, Albert K.
AU - Chamuleau, Robert A. F. M.
PY - 2002
Y1 - 2002
N2 - Mice lacking ApoA-V, a novel HDL-associated apolipoprotein identified by our group and independently by Permacchio et al. [Science 294 (2001) 169], were recently shown to be hypertriglyceridemic. To study the role of ApoA-V in triglyceride homeostasis, we compared lipid profiles in mice expressing normal and highly elevated levels of ApoA-V. For this purpose, adenoviral vectors expressing sense or antisense ApoA-V cDNA were constructed. Treatment of mice with sense adenoviral constructs resulted in circa 20-fold higher serum ApoA-V levels compared with mice injected with either PBS or antisense adenoviral constructs. ApoA-V overexpressing mice had markedly decreased (-70%) serum triglyceride levels caused primarily by lowered triglyceride content of the VLDL fraction. Furthermore, in these mice cholesterol levels were found to be lowered in all lipoprotein fractions with the largest mass decrease in the HDL fraction. This resulted in a 40% drop of serum cholesterol content. These findings suggest a role of ApoA-V in regulating levels of circulating triglycerides and cholesterol. (C) 2002 Elsevier Science (USA). All rights reserved
AB - Mice lacking ApoA-V, a novel HDL-associated apolipoprotein identified by our group and independently by Permacchio et al. [Science 294 (2001) 169], were recently shown to be hypertriglyceridemic. To study the role of ApoA-V in triglyceride homeostasis, we compared lipid profiles in mice expressing normal and highly elevated levels of ApoA-V. For this purpose, adenoviral vectors expressing sense or antisense ApoA-V cDNA were constructed. Treatment of mice with sense adenoviral constructs resulted in circa 20-fold higher serum ApoA-V levels compared with mice injected with either PBS or antisense adenoviral constructs. ApoA-V overexpressing mice had markedly decreased (-70%) serum triglyceride levels caused primarily by lowered triglyceride content of the VLDL fraction. Furthermore, in these mice cholesterol levels were found to be lowered in all lipoprotein fractions with the largest mass decrease in the HDL fraction. This resulted in a 40% drop of serum cholesterol content. These findings suggest a role of ApoA-V in regulating levels of circulating triglycerides and cholesterol. (C) 2002 Elsevier Science (USA). All rights reserved
U2 - https://doi.org/10.1016/S0006-291X(02)00808-2
DO - https://doi.org/10.1016/S0006-291X(02)00808-2
M3 - Article
C2 - 12135615
SN - 0006-291X
VL - 295
SP - 1156
EP - 1159
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 5
ER -