An analysis of the sequence variability of meningococcal fHbp, NadA and NHBA over a 50-year period in the Netherlands

Stefania Bambini; Jurgen Piet; Alessandro Muzzi; Wendy Keijzers; Sara Comandi; Lisa de Tora; Mariagrazia Pizza; Rino Rappuoli; Diederik van de Beek; Arie van der Ende; Maurizio Comanducci

doi:https://doi.org/10.1371/journal.pone.0065043

An analysis of the sequence variability of meningococcal fHbp, NadA and NHBA over a 50-year period in the Netherlands

Stefania Bambini, Jurgen Piet, Alessandro Muzzi, Wendy Keijzers, Sara Comandi, Lisa de Tora, Mariagrazia Pizza, Rino Rappuoli, Diederik van de Beek, Arie van der Ende, Maurizio Comanducci

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Abstract

Studies of meningococcal evolution and genetic population structure, including the long-term stability of non-random associations between variants of surface proteins, are essential for vaccine development. We analyzed the sequence variability of factor H-binding protein (fHbp), Neisserial Heparin-Binding Antigen (NHBA) and Neisseria adhesin A (NadA), three major antigens in the multicomponent meningococcal serogroup B vaccine 4CMenB. A panel of invasive isolates collected in the Netherlands over a period of 50 years was used. To our knowledge, this strain collection covers the longest time period of any collection available worldwide. Long-term persistence of several antigen sub/variants and of non-overlapping antigen sub/variant combinations was observed. Our data suggest that certain antigen sub/variants including those used in 4CMenB are conserved over time and promoted by selection

Original language	English
Pages (from-to)	e65043
Journal	PLOS ONE
Volume	8
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0065043
Publication status	Published - 2013

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https://doi.org/10.1371/journal.pone.0065043

Cite this

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title = "An analysis of the sequence variability of meningococcal fHbp, NadA and NHBA over a 50-year period in the Netherlands",

abstract = "Studies of meningococcal evolution and genetic population structure, including the long-term stability of non-random associations between variants of surface proteins, are essential for vaccine development. We analyzed the sequence variability of factor H-binding protein (fHbp), Neisserial Heparin-Binding Antigen (NHBA) and Neisseria adhesin A (NadA), three major antigens in the multicomponent meningococcal serogroup B vaccine 4CMenB. A panel of invasive isolates collected in the Netherlands over a period of 50 years was used. To our knowledge, this strain collection covers the longest time period of any collection available worldwide. Long-term persistence of several antigen sub/variants and of non-overlapping antigen sub/variant combinations was observed. Our data suggest that certain antigen sub/variants including those used in 4CMenB are conserved over time and promoted by selection",

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T1 - An analysis of the sequence variability of meningococcal fHbp, NadA and NHBA over a 50-year period in the Netherlands

AU - Bambini, Stefania

AU - Piet, Jurgen

AU - Muzzi, Alessandro

AU - Keijzers, Wendy

AU - Comandi, Sara

AU - de Tora, Lisa

AU - Pizza, Mariagrazia

AU - Rappuoli, Rino

AU - van de Beek, Diederik

AU - van der Ende, Arie

AU - Comanducci, Maurizio

PY - 2013

Y1 - 2013

N2 - Studies of meningococcal evolution and genetic population structure, including the long-term stability of non-random associations between variants of surface proteins, are essential for vaccine development. We analyzed the sequence variability of factor H-binding protein (fHbp), Neisserial Heparin-Binding Antigen (NHBA) and Neisseria adhesin A (NadA), three major antigens in the multicomponent meningococcal serogroup B vaccine 4CMenB. A panel of invasive isolates collected in the Netherlands over a period of 50 years was used. To our knowledge, this strain collection covers the longest time period of any collection available worldwide. Long-term persistence of several antigen sub/variants and of non-overlapping antigen sub/variant combinations was observed. Our data suggest that certain antigen sub/variants including those used in 4CMenB are conserved over time and promoted by selection

AB - Studies of meningococcal evolution and genetic population structure, including the long-term stability of non-random associations between variants of surface proteins, are essential for vaccine development. We analyzed the sequence variability of factor H-binding protein (fHbp), Neisserial Heparin-Binding Antigen (NHBA) and Neisseria adhesin A (NadA), three major antigens in the multicomponent meningococcal serogroup B vaccine 4CMenB. A panel of invasive isolates collected in the Netherlands over a period of 50 years was used. To our knowledge, this strain collection covers the longest time period of any collection available worldwide. Long-term persistence of several antigen sub/variants and of non-overlapping antigen sub/variant combinations was observed. Our data suggest that certain antigen sub/variants including those used in 4CMenB are conserved over time and promoted by selection

U2 - https://doi.org/10.1371/journal.pone.0065043

DO - https://doi.org/10.1371/journal.pone.0065043

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