TY - JOUR
T1 - Analysis of stem-cell-like properties of human CD161(++)IL-18R alpha(+) memory CD8(+) T cells
AU - Havenith, Simone H. C.
AU - Yong, Si La
AU - Henson, Sian M.
AU - Piet, Berber
AU - Idu, Mirza M.
AU - Koch, Sven D.
AU - Jonkers, René E.
AU - Kragten, Natasja A. M.
AU - Akbar, Arne N.
AU - van Lier, René A. W.
AU - ten Berge, Ineke J. M.
PY - 2012
Y1 - 2012
N2 - CD161(++)IL-18R(+)CD8(+) human T cells have recently been identified as a new subset of memory cells but their exact role remains unclear. CD161(++)IL-18R(+)CD8(+), mucosal-associated invariant T cells express a semi-invariant TCR V7.2-J33, which recognizes the MHC-related protein 1. On the basis of properties including the expression of the ABC-B1 transporter, cKit expression and survival after chemotherapy, CD161(++)IL-18R(+)CD8(+) T cells have been designated as ostem' cells. Here we analyse location and functional properties of CD161(++)IL-18R(+) CD8(+) T cells and question whether they have other traits that would mark them as genuine ostem' cells. CD161(++)IL-18R(+)CD8(+) T cells were found in peripheral blood, spleen and bone marrow but interestingly hardly at all in lymph nodes (LNs), which may possibly be explained by the finding that these cells express a specific set of chemokine receptors that allows migration to inflamed tissue rather than to LNs. In addition to TCR ligation and co-stimulation, CD161(++)IL-18R(+) CD8(+) T cells require cytokines for proliferation. The CD161(++)IL-18R(+) CD8(+) pool contains cells reactive towards peptides, derived from both persisting and cleared viruses. Although CD161(++)IL-18R(+) CD8(+) T cells express the ABC-B1 transporter, they have shorter telomeres and less telomerase activity and do not express aldehyde dehydrogenase. Finally, CD161(++)IL-18R(+) CD8(+) T cells show similarities to terminally differentiated T cells, expressing IFN, KLRG1 and the transcription factor Blimp-1. In conclusion, CD161(++)IL-18R(+) CD8(+) T cells lack many features of typical ostem' cells, but appear rather to be a subset of effector-type cells
AB - CD161(++)IL-18R(+)CD8(+) human T cells have recently been identified as a new subset of memory cells but their exact role remains unclear. CD161(++)IL-18R(+)CD8(+), mucosal-associated invariant T cells express a semi-invariant TCR V7.2-J33, which recognizes the MHC-related protein 1. On the basis of properties including the expression of the ABC-B1 transporter, cKit expression and survival after chemotherapy, CD161(++)IL-18R(+)CD8(+) T cells have been designated as ostem' cells. Here we analyse location and functional properties of CD161(++)IL-18R(+) CD8(+) T cells and question whether they have other traits that would mark them as genuine ostem' cells. CD161(++)IL-18R(+)CD8(+) T cells were found in peripheral blood, spleen and bone marrow but interestingly hardly at all in lymph nodes (LNs), which may possibly be explained by the finding that these cells express a specific set of chemokine receptors that allows migration to inflamed tissue rather than to LNs. In addition to TCR ligation and co-stimulation, CD161(++)IL-18R(+) CD8(+) T cells require cytokines for proliferation. The CD161(++)IL-18R(+) CD8(+) pool contains cells reactive towards peptides, derived from both persisting and cleared viruses. Although CD161(++)IL-18R(+) CD8(+) T cells express the ABC-B1 transporter, they have shorter telomeres and less telomerase activity and do not express aldehyde dehydrogenase. Finally, CD161(++)IL-18R(+) CD8(+) T cells show similarities to terminally differentiated T cells, expressing IFN, KLRG1 and the transcription factor Blimp-1. In conclusion, CD161(++)IL-18R(+) CD8(+) T cells lack many features of typical ostem' cells, but appear rather to be a subset of effector-type cells
U2 - https://doi.org/10.1093/intimm/dxs069
DO - https://doi.org/10.1093/intimm/dxs069
M3 - Article
C2 - 22836020
SN - 0953-8178
VL - 24
SP - 625
EP - 636
JO - International Immunology
JF - International Immunology
IS - 10
ER -