TY - JOUR
T1 - Borrelia miyamotoi Activates Human Dendritic Cells and Elicits T Cell Responses
AU - Mason, Lauren M. K.
AU - Koetsveld, Joris
AU - Trentelman, Jos J. A.
AU - Kaptein, Tanja M.
AU - Hoornstra, Dieuwertje
AU - Wagemakers, Alex
AU - Fikrig, Michelle M.
AU - Ersoz, Jasmin I.
AU - Oei, Anneke
AU - Geijtenbeek, Teunis B. H.
AU - Hovius, Joppe W. R.
PY - 2020
Y1 - 2020
N2 - The spirochete Borrelia miyamotoi has recently been shown to cause relapsing fever. Like the Lyme disease agent, Borrelia burgdorferi, B. miyamotoi is transmitted through the bite of infected ticks; however, little is known about the response of the immune system upon infection. Dendritic cells (DCs) play a central role in the early immune response against B. burgdorferi. We investigated the response of DCs to two different strains of B. miyamotoi using in vitro and ex vivo models and compared this to the response elicited by B. burgdorferi. Our findings show that B. miyamotoi is phagocytosed by monocyte-derived DCs, causing upregulation of activation markers and production of proinflammatory cytokines in a similar manner to B. burgdorferi. Recognition of B. miyamotoi was demonstrated to be partially mediated by TLR2. DCs migrated out of human skin explants upon inoculation of the skin with B. miyamotoi. Finally, we showed that B. miyamotoi–stimulated DCs induced proliferation of naive CD4 + and CD8 + T cells to a larger extent than B. burgdorferi. In conclusion, we show in this study that DCs respond to and mount an immune response against B. miyamotoi that is similar to the response to B. burgdorferi and is able to induce T cell proliferation. The Journal of Immunology, 2020, 204: 386–393.
AB - The spirochete Borrelia miyamotoi has recently been shown to cause relapsing fever. Like the Lyme disease agent, Borrelia burgdorferi, B. miyamotoi is transmitted through the bite of infected ticks; however, little is known about the response of the immune system upon infection. Dendritic cells (DCs) play a central role in the early immune response against B. burgdorferi. We investigated the response of DCs to two different strains of B. miyamotoi using in vitro and ex vivo models and compared this to the response elicited by B. burgdorferi. Our findings show that B. miyamotoi is phagocytosed by monocyte-derived DCs, causing upregulation of activation markers and production of proinflammatory cytokines in a similar manner to B. burgdorferi. Recognition of B. miyamotoi was demonstrated to be partially mediated by TLR2. DCs migrated out of human skin explants upon inoculation of the skin with B. miyamotoi. Finally, we showed that B. miyamotoi–stimulated DCs induced proliferation of naive CD4 + and CD8 + T cells to a larger extent than B. burgdorferi. In conclusion, we show in this study that DCs respond to and mount an immune response against B. miyamotoi that is similar to the response to B. burgdorferi and is able to induce T cell proliferation. The Journal of Immunology, 2020, 204: 386–393.
UR - http://www.scopus.com/inward/record.url?scp=85077669978&partnerID=8YFLogxK
U2 - https://doi.org/10.4049/jimmunol.1801589
DO - https://doi.org/10.4049/jimmunol.1801589
M3 - Article
C2 - 31818980
SN - 0022-1767
VL - 204
SP - 386
EP - 393
JO - Journal of immunology (Baltimore, Md.
JF - Journal of immunology (Baltimore, Md.
IS - 2
ER -