Activities per year
Abstract
Cell migration is centralto evoking a potent immune response. Dendritic cell (DC) migration to lymph nodes is dependent on the interaction of C-type lectin-like receptor 2 (CLEC-2; encoded by the gene Clec1b), expressed by DCs, with podoplanin, expressed by lymph node stromal cells, although the underlying molecular mechanisms remain elusive. Here, we show that CLEC-2-dependent DC migration is controlled by tetraspanin CD37, a membrane-organizing protein. We identified a specific interaction between CLEC-2 and CD37, and myeloid cells lacking CD37 (Cd37-/-) expressed reduced surface CLEC-2. CLEC-2-expressing Cd37-/- DCs showed impaired adhesion, migration velocity and displacementonlymph nodestromal cells. Moreover, Cd37-/- DCs failedto form actin protrusions in a 3Dcollagen matrix upon podoplanin-induced CLEC-2 stimulation, phenocopying CLEC-2-deficient DCs. Microcontact printing experiments revealed that CD37 is required for CLEC-2 recruitment in the membrane to its ligand podoplanin. Finally, Cd37-/- DCs failed to inhibit actomyosin contractility in lymph node stromal cells, thus phenocopying CLEC-2-deficient DCs. This study demonstrates that tetraspanin CD37 controls CLEC-2 membrane organization and provides new molecular insights into the mechanisms underlying CLEC-2-dependent DC migration. This article has an associated First Person interview with the first author of the paper.
Original language | English |
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Article number | jcs.214551 |
Journal | Journal of Cell Science |
Volume | 131 |
Issue number | 19 |
DOIs | |
Publication status | Published - 1 Oct 2018 |
Externally published | Yes |
Keywords
- CLEC-2
- Dendritic cell
- Membrane organization
- Migration
- Tetraspanin
Activities
- 1 Invited talk
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A pivotal role for lymph node stromal cells in orchestrating immunity
Lotte de Winde (Speaker)
15 Jan 2021Activity: Talk or presentation › Invited talk › Academic