TY - JOUR
T1 - Circulating MicroRNAs Characterizing Patients with Insufficient Coronary Collateral Artery Function
AU - Hakimzadeh, Nazanin
AU - Nossent, A. Yaël
AU - van der Laan, Anja M.
AU - Schirmer, Stephan H.
AU - de Ronde, Maurice W. J.
AU - Pinto-Sietsma, Sara-Joan
AU - van Royen, Niels
AU - Quax, Paul H. A.
AU - Hoefer, Imo E.
AU - Piek, Jan J.
PY - 2015
Y1 - 2015
N2 - Coronary collateral arteries function as natural bypasses in the event of coronary obstruction. The degree of collateral network development significantly impacts the outcome of patients after an acute myocardial infarction (AMI). MicroRNAs (miRNAs, miRs) have arisen as biomarkers to identify heterogeneous patients, as well as new therapeutic targets in cardiovascular disease. We sought to identify miRNAs that are differentially expressed in chronic total occlusion (CTO) patients with well or poorly developed collateral arteries. Forty-one CTO patients undergoing coronary angiography and invasive assessment of their coronary collateralization were dichotomized based on their collateral flow index (CFI). After miRNA profiling was conducted on aortic plasma, four miRNAs were selected for validation by real-time quantitative reverse transcription polymerase chain reaction in patients with low (CFI <0.39) and high (CFI>0.39) collateral artery capacity. We confirmed significantly elevated levels of miR423-5p (p <0.05), miR10b (p <0.05), miR30d (p <0.05) and miR126 (p <0.001) in patients with insufficient collateral network development. We further demonstrated that each of these miRNAs could serve as circulating biomarkers to discriminate patients with low collateral capacity (p <0.01 for each miRNA). We also determined significantly greater expression of miR30d (p <0.05) and miR126 (p <0.001) in CTO patients relative to healthy controls. The present study identifies differentially expressed miRNAs in patients with high versus low coronary collateral capacity. We have shown that these miRNAs can function as circulating biomarkers to discriminate between patients with insufficient or sufficient collateralization. This is the first study to identify miRNAs linked to coronary collateral vessel function in humans
AB - Coronary collateral arteries function as natural bypasses in the event of coronary obstruction. The degree of collateral network development significantly impacts the outcome of patients after an acute myocardial infarction (AMI). MicroRNAs (miRNAs, miRs) have arisen as biomarkers to identify heterogeneous patients, as well as new therapeutic targets in cardiovascular disease. We sought to identify miRNAs that are differentially expressed in chronic total occlusion (CTO) patients with well or poorly developed collateral arteries. Forty-one CTO patients undergoing coronary angiography and invasive assessment of their coronary collateralization were dichotomized based on their collateral flow index (CFI). After miRNA profiling was conducted on aortic plasma, four miRNAs were selected for validation by real-time quantitative reverse transcription polymerase chain reaction in patients with low (CFI <0.39) and high (CFI>0.39) collateral artery capacity. We confirmed significantly elevated levels of miR423-5p (p <0.05), miR10b (p <0.05), miR30d (p <0.05) and miR126 (p <0.001) in patients with insufficient collateral network development. We further demonstrated that each of these miRNAs could serve as circulating biomarkers to discriminate patients with low collateral capacity (p <0.01 for each miRNA). We also determined significantly greater expression of miR30d (p <0.05) and miR126 (p <0.001) in CTO patients relative to healthy controls. The present study identifies differentially expressed miRNAs in patients with high versus low coronary collateral capacity. We have shown that these miRNAs can function as circulating biomarkers to discriminate between patients with insufficient or sufficient collateralization. This is the first study to identify miRNAs linked to coronary collateral vessel function in humans
U2 - https://doi.org/10.1371/journal.pone.0137035
DO - https://doi.org/10.1371/journal.pone.0137035
M3 - Article
C2 - 26331273
SN - 1932-6203
VL - 10
SP - e0137035
JO - PLOS ONE
JF - PLOS ONE
IS - 9
M1 - e0137035
ER -