TY - JOUR
T1 - Connective tissue growth factor is necessary for retinal capillary basal lamina thickening in diabetic mice
AU - Kuiper, Esther J.
AU - van Zijderveld, Rogier
AU - Roestenberg, Peggy
AU - Lyons, Karen M.
AU - Goldschmeding, Roel
AU - Klaassen, Ingeborg
AU - van Noorden, Cornelis J. F.
AU - Schlingemann, Reinier O.
PY - 2008
Y1 - 2008
N2 - Experimental prevention of basal lamina (BL) thickening of retinal capillaries ameliorates early vascular changes caused by diabetes. Connective tissue growth factor (CTGF) is upregulated early in diabetes in the human retina and is a potent inducer of expression of BL components. We hypothesize that CTGF is causally involved in diabetes-induced BL thickening of retinal capillaries. To test this hypothesis, we compared the effects of streptozotocin (STZ)-induced diabetes on retinal capillary BL thickness between wild-type mice (CTGF(+/+)) and mice lacking one functional CTGF allele (CTGF(+/-)). Differences in BL thickness were calculated by quantitative analysis of electron microscopic images of transversally sectioned capillaries in and around the inner nuclear layer of the retina. We show that BL thickening was significant in diabetic CTGF(+/+) mice compared with control CTGF(+/+) mice, whereas diabetes did not significantly induce BL thickening in CTGF(+/-) mice. We conclude that CTGF expression is necessary for diabetes-induced BL thickening and suggest that reduction of CTGF levels may be protective against the development of diabetic retinopathy
AB - Experimental prevention of basal lamina (BL) thickening of retinal capillaries ameliorates early vascular changes caused by diabetes. Connective tissue growth factor (CTGF) is upregulated early in diabetes in the human retina and is a potent inducer of expression of BL components. We hypothesize that CTGF is causally involved in diabetes-induced BL thickening of retinal capillaries. To test this hypothesis, we compared the effects of streptozotocin (STZ)-induced diabetes on retinal capillary BL thickness between wild-type mice (CTGF(+/+)) and mice lacking one functional CTGF allele (CTGF(+/-)). Differences in BL thickness were calculated by quantitative analysis of electron microscopic images of transversally sectioned capillaries in and around the inner nuclear layer of the retina. We show that BL thickening was significant in diabetic CTGF(+/+) mice compared with control CTGF(+/+) mice, whereas diabetes did not significantly induce BL thickening in CTGF(+/-) mice. We conclude that CTGF expression is necessary for diabetes-induced BL thickening and suggest that reduction of CTGF levels may be protective against the development of diabetic retinopathy
U2 - https://doi.org/10.1369/jhc.2008.950980
DO - https://doi.org/10.1369/jhc.2008.950980
M3 - Article
C2 - 18474939
SN - 0022-1554
VL - 56
SP - 785
EP - 792
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
IS - 8
ER -