TY - JOUR
T1 - Critical assessment of human antibody generation in humanized mouse models
AU - Villaudy, Julien
AU - Schotte, Remko
AU - Legrand, Nicolas
AU - Spits, Hergen
PY - 2014
Y1 - 2014
N2 - Immunodeficient mice reconstituted with human hematopoietic stem cells provide a small-animal model for the study of development and function of human hematopoietic cells in vivo. However, in the current models, the immune response, and especially the humoral response by the human immune cells is far from optimal. The B cells found in these mice exhibit an immature and abnormal phenotype correlating with a reduced capacity to produce antigen-specific affinity matured antibodies upon infection or immunization. Herein, we review the current state of knowledge of development, function and antibody production of human B cells and discuss the obstacles for the improvement of these models. (C) 2014 Elsevier B.V. All rights reserved
AB - Immunodeficient mice reconstituted with human hematopoietic stem cells provide a small-animal model for the study of development and function of human hematopoietic cells in vivo. However, in the current models, the immune response, and especially the humoral response by the human immune cells is far from optimal. The B cells found in these mice exhibit an immature and abnormal phenotype correlating with a reduced capacity to produce antigen-specific affinity matured antibodies upon infection or immunization. Herein, we review the current state of knowledge of development, function and antibody production of human B cells and discuss the obstacles for the improvement of these models. (C) 2014 Elsevier B.V. All rights reserved
U2 - https://doi.org/10.1016/j.jim.2014.06.010
DO - https://doi.org/10.1016/j.jim.2014.06.010
M3 - Review article
C2 - 24952244
SN - 0022-1759
VL - 410
SP - 18
EP - 27
JO - Journal of immunological methods
JF - Journal of immunological methods
ER -