TY - JOUR
T1 - CXCR5+CD4+ follicular helper T cells accumulate in resting human lymph nodes and have superior B cell helper activity
AU - Havenith, Simone H. C.
AU - Remmerswaal, Ester B. M.
AU - Idu, Mirza M.
AU - van Donselaar-van der Pant, Karlijn A. M. I.
AU - van der Bom, Nelly
AU - Bemelman, Fréderike J.
AU - van Leeuwen, Ester M. M.
AU - ten Berge, Ineke J. M.
AU - van Lier, René A. W.
PY - 2014
Y1 - 2014
N2 - Although many relevant immune reactions are initiated in the lymph nodes, this compartment has not been systematically studied in humans. Analyses have been performed on immune cells derived from tonsils, but as this tissue is most often inflamed, generalization of these data is difficult. Here, we analyzed the phenotype and function of the human CD4(+) T-cell subsets and lineages in paired resting lymph node and peripheral blood samples. Naive, central memory cells and effector memory cells as well as Th1, Th2, Th17 and Treg cells were equally represented in both compartments. On the other hand, cytotoxic CD4(+) T cells were strikingly absent in the lymph nodes. CXCR5(+)CD4(+) T cells, representing putative follicular Th (Tfh) cells were over-represented in lymph nodes and expressed higher levels of Tfh markers than their peripheral blood counterparts. Compared with the circulating pool, lymph-node-derived CXCR5(+)CD4(+) T cells were superior in providing help to B cells. Thus, functionally competent Tfh cells accumulate in resting human lymph nodes, providing a swift induction of naive and memory antibody responses upon antigenic challenge
AB - Although many relevant immune reactions are initiated in the lymph nodes, this compartment has not been systematically studied in humans. Analyses have been performed on immune cells derived from tonsils, but as this tissue is most often inflamed, generalization of these data is difficult. Here, we analyzed the phenotype and function of the human CD4(+) T-cell subsets and lineages in paired resting lymph node and peripheral blood samples. Naive, central memory cells and effector memory cells as well as Th1, Th2, Th17 and Treg cells were equally represented in both compartments. On the other hand, cytotoxic CD4(+) T cells were strikingly absent in the lymph nodes. CXCR5(+)CD4(+) T cells, representing putative follicular Th (Tfh) cells were over-represented in lymph nodes and expressed higher levels of Tfh markers than their peripheral blood counterparts. Compared with the circulating pool, lymph-node-derived CXCR5(+)CD4(+) T cells were superior in providing help to B cells. Thus, functionally competent Tfh cells accumulate in resting human lymph nodes, providing a swift induction of naive and memory antibody responses upon antigenic challenge
U2 - https://doi.org/10.1093/intimm/dxt058
DO - https://doi.org/10.1093/intimm/dxt058
M3 - Article
C2 - 24291746
SN - 0953-8178
VL - 26
SP - 183
EP - 192
JO - International Immunology
JF - International Immunology
IS - 3
ER -