Cytomegalovirus early and late membrane antigens detected by antibodies in human convalescent sera

J M Middeldorp, J Jongsma, T H The

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Using indirect immunofluorescence and a panel of human convalescent-phase sera, we identified cytomegalovirus (CMV) early and late membrane antigens (CMV-EMA and CMV-LMA, respectively) as separate entities on the surfaces of viable CMV-infected fibroblasts starting at 6 to 12 and 36 to 48 h postinoculation, respectively. For expression of CMV-EMA and CMV-LMA, infectious virus and active protein synthesis were required, whereas the expression of CMV-LMA, in addition, required viral DNA synthesis. Our data suggest that CMV-EMA and CMV-LMA form an individual set of CMV antigens that are different from intracellular CMV antigens and possibly (partly) different from the viral envelope.

Original languageEnglish
Pages (from-to)240-4
Number of pages5
JournalJournal of Virology
Issue number1
Publication statusPublished - Apr 1985


  • Antibodies, Viral
  • Antigens, Viral
  • Cell Membrane
  • Cells, Cultured
  • Cytomegalovirus
  • Cytomegalovirus Infections
  • Fluorescent Antibody Technique
  • Humans
  • Journal Article
  • Membrane Proteins
  • Research Support, Non-U.S. Gov't
  • Time Factors

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