Abstract
Using indirect immunofluorescence and a panel of human convalescent-phase sera, we identified cytomegalovirus (CMV) early and late membrane antigens (CMV-EMA and CMV-LMA, respectively) as separate entities on the surfaces of viable CMV-infected fibroblasts starting at 6 to 12 and 36 to 48 h postinoculation, respectively. For expression of CMV-EMA and CMV-LMA, infectious virus and active protein synthesis were required, whereas the expression of CMV-LMA, in addition, required viral DNA synthesis. Our data suggest that CMV-EMA and CMV-LMA form an individual set of CMV antigens that are different from intracellular CMV antigens and possibly (partly) different from the viral envelope.
| Original language | English |
|---|---|
| Pages (from-to) | 240-4 |
| Number of pages | 5 |
| Journal | Journal of Virology |
| Volume | 54 |
| Issue number | 1 |
| Publication status | Published - Apr 1985 |
Keywords
- Antibodies, Viral
- Antigens, Viral
- Cell Membrane
- Cells, Cultured
- Cytomegalovirus
- Cytomegalovirus Infections
- Fluorescent Antibody Technique
- Humans
- Journal Article
- Membrane Proteins
- Research Support, Non-U.S. Gov't
- Time Factors