TY - JOUR
T1 - Decreased interleukin-10 and increased interleukin- 12p40 mRNA are associated with disease activity and characterize different disease stages in multiple sclerosis
AU - Van Boxel-Dezaire, A. H.H.
AU - Hoff, S. C.J.
AU - Van Oosten, B. W.
AU - Verweij, C. L.
AU - Dräger, A. M.
AU - Adèr, H. J.
AU - Van Houwelingen, J. C.
AU - Barkhof, F.
AU - Polman, C. H.
AU - Nagelkerken, L.
PY - 1999/6/15
Y1 - 1999/6/15
N2 - It has been shown that proinflammatory and antiinflammatory cytokines correlate with disease activity in multiple sclerosis (MS). To establish whether such correlations depend on the disease stage, we assessed in a longitudinal fashion the expression of interleukin (IL)-12 (p40 and p35), tumor necrosis factor- 1/4 , interferon-γ, and IL-10 mRNA by competitive polymerase chain reaction in unstimulated peripheral blood mononuclear cells of relapsing-remitting (RR) and secondary progressive (SP) MS patients, in relation to monthly clinical and magnetic resonance imaging monitoring. MS patients had increased levels of IL-12p40 and decreased levels of IL-10 mRNA compared with controls; this difference was most pronounced in SP patients. Both RR and SP patients had increased levels of IL-12p40 mRNA compared with controls during the development of active lesions. Moreover, in RR MS an increase was found before relapse. IL-12p35 mRNA was decreased in both groups, and in relation to disease activity it showed a pattern different from IL-12p40 mRNA. In RR MS, IL-10 mRNA was low 4 weeks before magnetic resonance imaging activity and 6 weeks before relapse; a significant increase to normal levels was noted when active lesions became apparent. In contrast, SP patients showed low IL-10 mRNA levels constitutively, suggesting that IL- 10 plays an important role in the control of disease progression.
AB - It has been shown that proinflammatory and antiinflammatory cytokines correlate with disease activity in multiple sclerosis (MS). To establish whether such correlations depend on the disease stage, we assessed in a longitudinal fashion the expression of interleukin (IL)-12 (p40 and p35), tumor necrosis factor- 1/4 , interferon-γ, and IL-10 mRNA by competitive polymerase chain reaction in unstimulated peripheral blood mononuclear cells of relapsing-remitting (RR) and secondary progressive (SP) MS patients, in relation to monthly clinical and magnetic resonance imaging monitoring. MS patients had increased levels of IL-12p40 and decreased levels of IL-10 mRNA compared with controls; this difference was most pronounced in SP patients. Both RR and SP patients had increased levels of IL-12p40 mRNA compared with controls during the development of active lesions. Moreover, in RR MS an increase was found before relapse. IL-12p35 mRNA was decreased in both groups, and in relation to disease activity it showed a pattern different from IL-12p40 mRNA. In RR MS, IL-10 mRNA was low 4 weeks before magnetic resonance imaging activity and 6 weeks before relapse; a significant increase to normal levels was noted when active lesions became apparent. In contrast, SP patients showed low IL-10 mRNA levels constitutively, suggesting that IL- 10 plays an important role in the control of disease progression.
UR - http://www.scopus.com/inward/record.url?scp=0032975730&partnerID=8YFLogxK
U2 - https://doi.org/10.1002/1531-8249(199906)45:6<695::AID-ANA3>3.0.CO;2-R
DO - https://doi.org/10.1002/1531-8249(199906)45:6<695::AID-ANA3>3.0.CO;2-R
M3 - Article
C2 - 10360761
SN - 0364-5134
VL - 45
SP - 695
EP - 703
JO - Annals of neurology
JF - Annals of neurology
IS - 6
ER -