TY - JOUR
T1 - Dendritic cell type-specific HIV-1 activation in effector T cells
T2 - Implications for latent HIV-1 reservoir establishment
AU - Van Der Sluis, Reneé M.
AU - Van Capel, Toni M.M.
AU - Speijer, Dave
AU - Sanders, Rogier W.
AU - Berkhout, Ben
AU - De Jong, Esther C.
AU - Jeeninga, Rienk E.
AU - Van Montfort, Thijs
N1 - Publisher Copyright: © Copyright 2015 Wolters Kluwer Health, Inc. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Background: Latent HIV type I (HIV-1) infections can frequently occur in short-lived proliferating effector T lymphocytes. These latently infected cells could revert into resting T lymphocytes and thereby contribute to the establishment of the long-lived viral reservoir. Monocyte-derived dendritic cells can revert latency in effector T cells in vitro. Methods: Here we investigated the latency activation properties of tissue-specific immune cells, including a large panel of dendritic cell subsets, to explore in which body compartments effector T cells are most likely to maintain latent HIV-1 provirus and thus potentially contribute to the long-lived reservoir. Results: Our results demonstrate that blood or genital tract dendritic cells do not activate latent provirus in effector T cells, whereas gut or lymphoid dendritic cells induce virus production from latently infected effector T cells in our in-vitro model for latency. Toll-like receptor 3-induced interferon production by myeloid dendritic cells abolished the dendritic cells' ability to induce viral gene expression. Conclusions: In this study, we show that HIV-1 provirus residing in effector T cells is activated from latency by tissue-specific dendritic cell subsets and other immune cells with remarkably different efficiencies. Our new assay system points to an important, neglected aspect of HIV-1 research: the ability of other immune cells, especially dendritic cells, to differentially affect latency establishment as well as virus reactivation.
AB - Background: Latent HIV type I (HIV-1) infections can frequently occur in short-lived proliferating effector T lymphocytes. These latently infected cells could revert into resting T lymphocytes and thereby contribute to the establishment of the long-lived viral reservoir. Monocyte-derived dendritic cells can revert latency in effector T cells in vitro. Methods: Here we investigated the latency activation properties of tissue-specific immune cells, including a large panel of dendritic cell subsets, to explore in which body compartments effector T cells are most likely to maintain latent HIV-1 provirus and thus potentially contribute to the long-lived reservoir. Results: Our results demonstrate that blood or genital tract dendritic cells do not activate latent provirus in effector T cells, whereas gut or lymphoid dendritic cells induce virus production from latently infected effector T cells in our in-vitro model for latency. Toll-like receptor 3-induced interferon production by myeloid dendritic cells abolished the dendritic cells' ability to induce viral gene expression. Conclusions: In this study, we show that HIV-1 provirus residing in effector T cells is activated from latency by tissue-specific dendritic cell subsets and other immune cells with remarkably different efficiencies. Our new assay system points to an important, neglected aspect of HIV-1 research: the ability of other immune cells, especially dendritic cells, to differentially affect latency establishment as well as virus reactivation.
KW - Dendritic cell - T-cell interactions
KW - Dendritic cell disruption of HIV-1 latency
KW - Dendritic cell subsetspecific purging
KW - HIV-1
KW - HIV-1 latency establishment
KW - Reversion of HIV-1 latency
UR - http://www.scopus.com/inward/record.url?scp=84988723073&partnerID=8YFLogxK
U2 - https://doi.org/10.1097/QAD.0000000000000637
DO - https://doi.org/10.1097/QAD.0000000000000637
M3 - Article
C2 - 25768834
SN - 0269-9370
VL - 29
SP - 1003
EP - 1014
JO - AIDS
JF - AIDS
IS - 9
ER -