Design and use of conditional MHC class I ligands

Mireille Toebes; Miriam Coccoris; Adriaan Bins; Boris Rodenko; Raquel Gomez; Nella J. Nieuwkoop; Willeke van de Kasteele; Guus F. Rimmelzwaan; John B. A. G. Haanen; Huib Ovaa; Ton N. M. Schumacher

doi:https://doi.org/10.1038/nm1360

Design and use of conditional MHC class I ligands

Mireille Toebes, Miriam Coccoris, Adriaan Bins, Boris Rodenko, Raquel Gomez, Nella J. Nieuwkoop, Willeke van de Kasteele, Guus F. Rimmelzwaan, John B. A. G. Haanen, Huib Ovaa, Ton N. M. Schumacher

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Research output: Contribution to journal › Article › Academic › peer-review

274 Citations (Scopus)

Abstract

Major histocompatibility complex (MHC) class I molecules associate with a variety of peptide ligands during biosynthesis and present these ligands on the cell surface for recognition by cytotoxic T cells. We have designed conditional MHC ligands that form stable complexes with MHC molecules but degrade on command, by exposure to a defined photostimulus. 'Empty MHC molecules' generated in this manner can be loaded with arrays of peptide ligands to determine MHC binding properties and to monitor antigen-specific T-cell responses in a high-throughput manner. We document the value of this approach by identifying cytotoxic T-cell epitopes within the H5N1 influenza A/Vietnam/1194/04 genome

Original language	English
Pages (from-to)	246-251
Journal	Nature medicine
Volume	12
Issue number	2
DOIs	https://doi.org/10.1038/nm1360
Publication status	Published - 2006

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https://doi.org/10.1038/nm1360

Cite this

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title = "Design and use of conditional MHC class I ligands",

abstract = "Major histocompatibility complex (MHC) class I molecules associate with a variety of peptide ligands during biosynthesis and present these ligands on the cell surface for recognition by cytotoxic T cells. We have designed conditional MHC ligands that form stable complexes with MHC molecules but degrade on command, by exposure to a defined photostimulus. 'Empty MHC molecules' generated in this manner can be loaded with arrays of peptide ligands to determine MHC binding properties and to monitor antigen-specific T-cell responses in a high-throughput manner. We document the value of this approach by identifying cytotoxic T-cell epitopes within the H5N1 influenza A/Vietnam/1194/04 genome",

author = "Mireille Toebes and Miriam Coccoris and Adriaan Bins and Boris Rodenko and Raquel Gomez and Nieuwkoop, {Nella J.} and {van de Kasteele}, Willeke and Rimmelzwaan, {Guus F.} and Haanen, {John B. A. G.} and Huib Ovaa and Schumacher, {Ton N. M.}",

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T1 - Design and use of conditional MHC class I ligands

AU - Toebes, Mireille

AU - Coccoris, Miriam

AU - Bins, Adriaan

AU - Rodenko, Boris

AU - Gomez, Raquel

AU - Nieuwkoop, Nella J.

AU - van de Kasteele, Willeke

AU - Rimmelzwaan, Guus F.

AU - Haanen, John B. A. G.

AU - Ovaa, Huib

AU - Schumacher, Ton N. M.

PY - 2006

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AB - Major histocompatibility complex (MHC) class I molecules associate with a variety of peptide ligands during biosynthesis and present these ligands on the cell surface for recognition by cytotoxic T cells. We have designed conditional MHC ligands that form stable complexes with MHC molecules but degrade on command, by exposure to a defined photostimulus. 'Empty MHC molecules' generated in this manner can be loaded with arrays of peptide ligands to determine MHC binding properties and to monitor antigen-specific T-cell responses in a high-throughput manner. We document the value of this approach by identifying cytotoxic T-cell epitopes within the H5N1 influenza A/Vietnam/1194/04 genome

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DO - https://doi.org/10.1038/nm1360

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C2 - 16462803

SN - 1078-8956

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EP - 251

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JF - Nature medicine

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