TY - JOUR
T1 - Differences in Inflammation Patterns Induced by African and Asian Burkholderia pseudomallei Isolates in Mice
AU - Weehuizen, Tassili A. F.
AU - Birnie, Emma
AU - Ferwerda, Bart
AU - Roelofs, Joris J. T. H.
AU - de Vos, Alex F.
AU - Grobusch, Martin P.
AU - Wiersinga, W. Joost
PY - 2017
Y1 - 2017
N2 - AbstractBurkholderia pseudomallei is the causative agent of melioidosis, an emerging tropical disease of high mortality. Sub-Saharan Africa represents potential melioidosis "hotspots"; however, to date, only a few cases have been reported. Here in, we compared the inflammatory patterns induced by a B. pseudomallei strain recently isolated from a fatal Gabonese case with the Thai reference strain B. pseudomallei-1026b and Burkholderia thailandensis-E264. Ex vivo, no differences were observed in terms of cellular responsiveness between strains. However, when compared with the B. pseudomallei-1026b strain, the Gabonese isolate was significantly less virulent in terms of bacterial dissemination, inflammatory response, and organ damage in mice. Genomic comparison between strains showed differences in regions containing a fimbriae/adhesion virulence protein. In addition to a lack of microbiology facilities, differences in virulence of Burkholderia strains might contribute to the diverse global clinical occurrence of melioidosis
AB - AbstractBurkholderia pseudomallei is the causative agent of melioidosis, an emerging tropical disease of high mortality. Sub-Saharan Africa represents potential melioidosis "hotspots"; however, to date, only a few cases have been reported. Here in, we compared the inflammatory patterns induced by a B. pseudomallei strain recently isolated from a fatal Gabonese case with the Thai reference strain B. pseudomallei-1026b and Burkholderia thailandensis-E264. Ex vivo, no differences were observed in terms of cellular responsiveness between strains. However, when compared with the B. pseudomallei-1026b strain, the Gabonese isolate was significantly less virulent in terms of bacterial dissemination, inflammatory response, and organ damage in mice. Genomic comparison between strains showed differences in regions containing a fimbriae/adhesion virulence protein. In addition to a lack of microbiology facilities, differences in virulence of Burkholderia strains might contribute to the diverse global clinical occurrence of melioidosis
U2 - https://doi.org/10.4269/ajtmh.16-0121
DO - https://doi.org/10.4269/ajtmh.16-0121
M3 - Article
C2 - 28719265
SN - 0002-9637
VL - 96
SP - 1365
EP - 1369
JO - American journal of tropical medicine and hygiene
JF - American journal of tropical medicine and hygiene
IS - 6
ER -