Epstein-Barr virus gene expression in oral hairy leukoplakia

R Lau; J Middeldorp; P J Farrell

doi:https://doi.org/10.1006/viro.1993.1397

Epstein-Barr virus gene expression in oral hairy leukoplakia

R Lau, J Middeldorp, P J Farrell

Research output: Contribution to journal › Article › Academic › peer-review

32 Citations (Scopus)

Abstract

A combination of Northern blotting and sequencing of clones from a cDNA library constructed using RNA isolated from oral hairy leukoplakia (OHL) has been used to study Epstein-Barr virus (EBV) gene expression in this AIDS-associated lesion, the only accessible source of in vivo-replicating EBV. Because of the limited amount of RNA available, Northern blotting was only useful for detection of very abundant EBV transcripts in the OHL biopsies. Analysis of cDNA clones containing the BdRF1, BCRF1, gp350/220, BARF0, and BKRF4 reading frames and further characterization of RNA structures spanning BCRF1 (viral IL-10) in lymphocytes infected with EBV has provided a preliminary comparison of some virus replicative gene expression in its two main host cell types. No expression of EBNA-1, EBNA-2, or EBNA-3A RNA was detected in the oral hairy leukoplakia cDNA library.

Original language	English
Pages (from-to)	463-74
Number of pages	12
Journal	Virology
Volume	195
Issue number	2
DOIs	https://doi.org/10.1006/viro.1993.1397
Publication status	Published - Aug 1993

Keywords

Animals
Base Sequence
Blotting, Northern
Blotting, Western
Cells, Cultured
DNA, Viral
Gene Expression
Gene Library
Genes, Viral
Herpesvirus 4, Human
Humans
Interleukin-10
Journal Article
Leukoplakia, Oral
Male
Mice
Mice, Nude
Molecular Sequence Data
RNA, Viral
Receptors, Complement 3d
Viral Proteins

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https://doi.org/10.1006/viro.1993.1397

Cite this

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title = "Epstein-Barr virus gene expression in oral hairy leukoplakia",

abstract = "A combination of Northern blotting and sequencing of clones from a cDNA library constructed using RNA isolated from oral hairy leukoplakia (OHL) has been used to study Epstein-Barr virus (EBV) gene expression in this AIDS-associated lesion, the only accessible source of in vivo-replicating EBV. Because of the limited amount of RNA available, Northern blotting was only useful for detection of very abundant EBV transcripts in the OHL biopsies. Analysis of cDNA clones containing the BdRF1, BCRF1, gp350/220, BARF0, and BKRF4 reading frames and further characterization of RNA structures spanning BCRF1 (viral IL-10) in lymphocytes infected with EBV has provided a preliminary comparison of some virus replicative gene expression in its two main host cell types. No expression of EBNA-1, EBNA-2, or EBNA-3A RNA was detected in the oral hairy leukoplakia cDNA library.",

keywords = "Animals, Base Sequence, Blotting, Northern, Blotting, Western, Cells, Cultured, DNA, Viral, Gene Expression, Gene Library, Genes, Viral, Herpesvirus 4, Human, Humans, Interleukin-10, Journal Article, Leukoplakia, Oral, Male, Mice, Mice, Nude, Molecular Sequence Data, RNA, Viral, Receptors, Complement 3d, Viral Proteins",

author = "R Lau and J Middeldorp and Farrell, {P J}",

year = "1993",

month = aug,

doi = "https://doi.org/10.1006/viro.1993.1397",

language = "English",

volume = "195",

pages = "463--74",

journal = "Virology",

issn = "0042-6822",

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AU - Lau, R

AU - Middeldorp, J

AU - Farrell, P J

PY - 1993/8

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N2 - A combination of Northern blotting and sequencing of clones from a cDNA library constructed using RNA isolated from oral hairy leukoplakia (OHL) has been used to study Epstein-Barr virus (EBV) gene expression in this AIDS-associated lesion, the only accessible source of in vivo-replicating EBV. Because of the limited amount of RNA available, Northern blotting was only useful for detection of very abundant EBV transcripts in the OHL biopsies. Analysis of cDNA clones containing the BdRF1, BCRF1, gp350/220, BARF0, and BKRF4 reading frames and further characterization of RNA structures spanning BCRF1 (viral IL-10) in lymphocytes infected with EBV has provided a preliminary comparison of some virus replicative gene expression in its two main host cell types. No expression of EBNA-1, EBNA-2, or EBNA-3A RNA was detected in the oral hairy leukoplakia cDNA library.

AB - A combination of Northern blotting and sequencing of clones from a cDNA library constructed using RNA isolated from oral hairy leukoplakia (OHL) has been used to study Epstein-Barr virus (EBV) gene expression in this AIDS-associated lesion, the only accessible source of in vivo-replicating EBV. Because of the limited amount of RNA available, Northern blotting was only useful for detection of very abundant EBV transcripts in the OHL biopsies. Analysis of cDNA clones containing the BdRF1, BCRF1, gp350/220, BARF0, and BKRF4 reading frames and further characterization of RNA structures spanning BCRF1 (viral IL-10) in lymphocytes infected with EBV has provided a preliminary comparison of some virus replicative gene expression in its two main host cell types. No expression of EBNA-1, EBNA-2, or EBNA-3A RNA was detected in the oral hairy leukoplakia cDNA library.

KW - Animals

KW - Base Sequence

KW - Blotting, Northern

KW - Blotting, Western

KW - Cells, Cultured

KW - DNA, Viral

KW - Gene Expression

KW - Gene Library

KW - Genes, Viral

KW - Herpesvirus 4, Human

KW - Humans

KW - Interleukin-10

KW - Journal Article

KW - Leukoplakia, Oral

KW - Male

KW - Mice

KW - Mice, Nude

KW - Molecular Sequence Data

KW - RNA, Viral

KW - Receptors, Complement 3d

KW - Viral Proteins

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DO - https://doi.org/10.1006/viro.1993.1397

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SN - 0042-6822

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SP - 463

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JO - Virology

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