TY - JOUR
T1 - Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells
AU - Boonkaew, Bootsakorn
AU - Thummavichit, Weeradee
AU - Netsrithong, Ratchapong
AU - Vatanashevanopakorn, Chinnavuth
AU - Pattanapanyasat, Kovit
AU - Wattanapanitch, Methichit
N1 - Funding Information: This research project was supported by the Thailand Research Fund (TRF) – Distinguished Research Professor Grant, contract no. DPG5980001 and IRG 5980006 , Siriraj Research Fund, Faculty of Medicine Siriraj Hospital, Mahidol University , Grant number (IO) R016033017 . MW and CV are supported by Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University. Publisher Copyright: © 2018 The Authors
PY - 2018/7
Y1 - 2018/7
N2 - Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders.
AB - Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders.
UR - http://www.scopus.com/inward/record.url?scp=85047008508&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.scr.2018.05.002
DO - https://doi.org/10.1016/j.scr.2018.05.002
M3 - Article
C2 - 29778975
SN - 1873-5061
VL - 30
SP - 34
EP - 37
JO - Stem Cell Research
JF - Stem Cell Research
ER -