EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations

A. W. Langerak; P. J. T. A. Groenen; M. Brüggemann; K. Beldjord; C. Bellan; L. Bonello; E. Boone; G. I. Carter; M. Catherwood; F. Davi; M.-H. Delfau-Larue; T. Diss; P. A. S. Evans; P. Gameiro; R. Garcia Sanz; D. Gonzalez; D. Grand; A. Håkansson; M. Hummel; H. Liu; L. Lombardia; E. A. Macintyre; B. J. Milner; S. Montes-Moreno; E. Schuuring; M. Spaargaren; E. Hodges; J. J. M. van Dongen

doi:https://doi.org/10.1038/leu.2012.246

EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations

A. W. Langerak, P. J. T. A. Groenen, M. Brüggemann, K. Beldjord, C. Bellan, L. Bonello, E. Boone, G. I. Carter, M. Catherwood, F. Davi, M.-H. Delfau-Larue, T. Diss, P. A. S. Evans, P. Gameiro, R. Garcia Sanz, D. Gonzalez, D. Grand, A. Håkansson, M. Hummel, H. LiuL. Lombardia, E. A. Macintyre, B. J. Milner, S. Montes-Moreno, E. Schuuring, M. Spaargaren, E. Hodges, J. J. M. van Dongen

Research output: Contribution to journal › Review article › Academic › peer-review

376 Citations (Scopus)

Abstract

PCR-based immunoglobulin (Ig)/T-cell receptor (TCR) clonality testing in suspected lymphoproliferations has largely been standardized and has consequently become technically feasible in a routine diagnostic setting. Standardization of the pre-analytical and post-analytical phases is now essential to prevent misinterpretation and incorrect conclusions derived from clonality data. As clonality testing is not a quantitative assay, but rather concerns recognition of molecular patterns, guidelines for reliable interpretation and reporting are mandatory. Here, the EuroClonality (BIOMED-2) consortium summarizes important pre- and post-analytical aspects of clonality testing, provides guidelines for interpretation of clonality testing results, and presents a uniform way to report the results of the Ig/TCR assays. Starting from an immunobiological concept, two levels to report Ig/TCR profiles are discerned: the technical description of individual (multiplex) PCR reactions and the overall molecular conclusion for B and T cells. Collectively, the EuroClonality (BIOMED-2) guidelines and consensus reporting system should help to improve the general performance level of clonality assessment and interpretation, which will directly impact on routine clinical management (standardized best-practice) in patients with suspected lymphoproliferations

Original language	English
Pages (from-to)	2159-2171
Journal	Leukemia
Volume	26
Issue number	10
DOIs	https://doi.org/10.1038/leu.2012.246
Publication status	Published - 2012

Access to Document

https://doi.org/10.1038/leu.2012.246

Cite this

Langerak, A. W., Groenen, P. J. T. A., Brüggemann, M., Beldjord, K., Bellan, C., Bonello, L., Boone, E., Carter, G. I., Catherwood, M., Davi, F., Delfau-Larue, M.-H., Diss, T., Evans, P. A. S., Gameiro, P., Garcia Sanz, R., Gonzalez, D., Grand, D., Håkansson, A., Hummel, M., ... van Dongen, J. J. M. (2012). EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations. Leukemia, 26(10), 2159-2171. https://doi.org/10.1038/leu.2012.246

@article{ba37ba8bac494224b6660f58d62c00f7,

title = "EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations",

abstract = "PCR-based immunoglobulin (Ig)/T-cell receptor (TCR) clonality testing in suspected lymphoproliferations has largely been standardized and has consequently become technically feasible in a routine diagnostic setting. Standardization of the pre-analytical and post-analytical phases is now essential to prevent misinterpretation and incorrect conclusions derived from clonality data. As clonality testing is not a quantitative assay, but rather concerns recognition of molecular patterns, guidelines for reliable interpretation and reporting are mandatory. Here, the EuroClonality (BIOMED-2) consortium summarizes important pre- and post-analytical aspects of clonality testing, provides guidelines for interpretation of clonality testing results, and presents a uniform way to report the results of the Ig/TCR assays. Starting from an immunobiological concept, two levels to report Ig/TCR profiles are discerned: the technical description of individual (multiplex) PCR reactions and the overall molecular conclusion for B and T cells. Collectively, the EuroClonality (BIOMED-2) guidelines and consensus reporting system should help to improve the general performance level of clonality assessment and interpretation, which will directly impact on routine clinical management (standardized best-practice) in patients with suspected lymphoproliferations",

author = "Langerak, {A. W.} and Groenen, {P. J. T. A.} and M. Br{\"u}ggemann and K. Beldjord and C. Bellan and L. Bonello and E. Boone and Carter, {G. I.} and M. Catherwood and F. Davi and M.-H. Delfau-Larue and T. Diss and Evans, {P. A. S.} and P. Gameiro and {Garcia Sanz}, R. and D. Gonzalez and D. Grand and A. H{\aa}kansson and M. Hummel and H. Liu and L. Lombardia and Macintyre, {E. A.} and Milner, {B. J.} and S. Montes-Moreno and E. Schuuring and M. Spaargaren and E. Hodges and {van Dongen}, {J. J. M.}",

year = "2012",

doi = "https://doi.org/10.1038/leu.2012.246",

language = "English",

volume = "26",

pages = "2159--2171",

journal = "Leukemia",

issn = "0887-6924",

publisher = "Nature Publishing Group",

number = "10",

}

Langerak, AW, Groenen, PJTA, Brüggemann, M, Beldjord, K, Bellan, C, Bonello, L, Boone, E, Carter, GI, Catherwood, M, Davi, F, Delfau-Larue, M-H, Diss, T, Evans, PAS, Gameiro, P, Garcia Sanz, R, Gonzalez, D, Grand, D, Håkansson, A, Hummel, M, Liu, H, Lombardia, L, Macintyre, EA, Milner, BJ, Montes-Moreno, S, Schuuring, E, Spaargaren, M, Hodges, E & van Dongen, JJM 2012, 'EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations', Leukemia, vol. 26, no. 10, pp. 2159-2171. https://doi.org/10.1038/leu.2012.246

TY - JOUR

T1 - EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations

AU - Langerak, A. W.

AU - Groenen, P. J. T. A.

AU - Brüggemann, M.

AU - Beldjord, K.

AU - Bellan, C.

AU - Bonello, L.

AU - Boone, E.

AU - Carter, G. I.

AU - Catherwood, M.

AU - Davi, F.

AU - Delfau-Larue, M.-H.

AU - Diss, T.

AU - Evans, P. A. S.

AU - Gameiro, P.

AU - Garcia Sanz, R.

AU - Gonzalez, D.

AU - Grand, D.

AU - Håkansson, A.

AU - Hummel, M.

AU - Liu, H.

AU - Lombardia, L.

AU - Macintyre, E. A.

AU - Milner, B. J.

AU - Montes-Moreno, S.

AU - Schuuring, E.

AU - Spaargaren, M.

AU - Hodges, E.

AU - van Dongen, J. J. M.

PY - 2012

Y1 - 2012

N2 - PCR-based immunoglobulin (Ig)/T-cell receptor (TCR) clonality testing in suspected lymphoproliferations has largely been standardized and has consequently become technically feasible in a routine diagnostic setting. Standardization of the pre-analytical and post-analytical phases is now essential to prevent misinterpretation and incorrect conclusions derived from clonality data. As clonality testing is not a quantitative assay, but rather concerns recognition of molecular patterns, guidelines for reliable interpretation and reporting are mandatory. Here, the EuroClonality (BIOMED-2) consortium summarizes important pre- and post-analytical aspects of clonality testing, provides guidelines for interpretation of clonality testing results, and presents a uniform way to report the results of the Ig/TCR assays. Starting from an immunobiological concept, two levels to report Ig/TCR profiles are discerned: the technical description of individual (multiplex) PCR reactions and the overall molecular conclusion for B and T cells. Collectively, the EuroClonality (BIOMED-2) guidelines and consensus reporting system should help to improve the general performance level of clonality assessment and interpretation, which will directly impact on routine clinical management (standardized best-practice) in patients with suspected lymphoproliferations

AB - PCR-based immunoglobulin (Ig)/T-cell receptor (TCR) clonality testing in suspected lymphoproliferations has largely been standardized and has consequently become technically feasible in a routine diagnostic setting. Standardization of the pre-analytical and post-analytical phases is now essential to prevent misinterpretation and incorrect conclusions derived from clonality data. As clonality testing is not a quantitative assay, but rather concerns recognition of molecular patterns, guidelines for reliable interpretation and reporting are mandatory. Here, the EuroClonality (BIOMED-2) consortium summarizes important pre- and post-analytical aspects of clonality testing, provides guidelines for interpretation of clonality testing results, and presents a uniform way to report the results of the Ig/TCR assays. Starting from an immunobiological concept, two levels to report Ig/TCR profiles are discerned: the technical description of individual (multiplex) PCR reactions and the overall molecular conclusion for B and T cells. Collectively, the EuroClonality (BIOMED-2) guidelines and consensus reporting system should help to improve the general performance level of clonality assessment and interpretation, which will directly impact on routine clinical management (standardized best-practice) in patients with suspected lymphoproliferations

U2 - https://doi.org/10.1038/leu.2012.246

DO - https://doi.org/10.1038/leu.2012.246

M3 - Review article

C2 - 22918122

SN - 0887-6924

VL - 26

SP - 2159

EP - 2171

JO - Leukemia

JF - Leukemia

IS - 10

ER -