TY - JOUR
T1 - Evaluation of the haemostatic potential of factor VIII-heparin cofactor II hybrid proteins in a mouse model
AU - Schatz, Simone
AU - Turecek, Peter L.
AU - Fiedler, Christian
AU - Zimmermann, Klaus
AU - Gritsch, Herbert
AU - Voorberg, Jan
AU - Schwarz, Hans Peter
AU - Dorner, Friedrich
AU - Scheiflinger, Friedrich
PY - 2003
Y1 - 2003
N2 - We constructed factor VIII-heparin cofactor II (FVIII-HCII) hybrid molecules, which are more readily activated by thrombin in vitro than the respective wild-type molecules. The hybrid proteins were tested in a murine model of haemophilia A to investigate their haemostatic efficacy in vivo. Bleeding characteristics, measured using standard tail-tip cutting techniques, were total blood loss, bleeding time and survival rate. FVIII-HCII hybrids were found to be effective in preventing bleeding in FVIII knockout mice. While in vitro experiments showed that the chimaeric molecules had higher haemostatic functions than the wild-type proteins, the variables analysed in vivo were similar for both proteins
AB - We constructed factor VIII-heparin cofactor II (FVIII-HCII) hybrid molecules, which are more readily activated by thrombin in vitro than the respective wild-type molecules. The hybrid proteins were tested in a murine model of haemophilia A to investigate their haemostatic efficacy in vivo. Bleeding characteristics, measured using standard tail-tip cutting techniques, were total blood loss, bleeding time and survival rate. FVIII-HCII hybrids were found to be effective in preventing bleeding in FVIII knockout mice. While in vitro experiments showed that the chimaeric molecules had higher haemostatic functions than the wild-type proteins, the variables analysed in vivo were similar for both proteins
U2 - https://doi.org/10.1046/j.1365-2141.2003.04674.x
DO - https://doi.org/10.1046/j.1365-2141.2003.04674.x
M3 - Article
C2 - 14616974
SN - 0007-1048
VL - 123
SP - 692
EP - 695
JO - British journal of haematology
JF - British journal of haematology
IS - 4
ER -