TY - JOUR
T1 - Experimental model for the study of the human immune system: production and monitoring of "human immune system" Rag2-/-gamma c-/- mice
AU - Legrand, Nicolas
AU - Weijer, Kees
AU - Spits, Hergen
PY - 2008
Y1 - 2008
N2 - Since the late 1980s, the study of the function and development of the human immune system has made intensive use of humanized animal models, among which mouse models have been proven extremely efficient and handy. Recent advances have lead to the establishment of new models with improved characteristics, both in terms of engraftment efficiency and in situ multilineage human hematopoietic development. In particular, the use of newborn BALB/c Rag2-/-gamma c-/- mice as recipients for human hematopoietic stem cells has proven particularly efficient. We describe here how to produce and monitor such "human immune system" (HIS) (BALB-Rag/gamma) mice, which offer large prospects for experimental study of the human immune system and as a preclinical screening tool
AB - Since the late 1980s, the study of the function and development of the human immune system has made intensive use of humanized animal models, among which mouse models have been proven extremely efficient and handy. Recent advances have lead to the establishment of new models with improved characteristics, both in terms of engraftment efficiency and in situ multilineage human hematopoietic development. In particular, the use of newborn BALB/c Rag2-/-gamma c-/- mice as recipients for human hematopoietic stem cells has proven particularly efficient. We describe here how to produce and monitor such "human immune system" (HIS) (BALB-Rag/gamma) mice, which offer large prospects for experimental study of the human immune system and as a preclinical screening tool
U2 - https://doi.org/10.1007/978-1-59745-570-1_4
DO - https://doi.org/10.1007/978-1-59745-570-1_4
M3 - Article
C2 - 18370148
SN - 1064-3745
VL - 415
SP - 65
EP - 82
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -