Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys

S L Li, H Feichtinger, E Kaaya, P Migliorini, P Putkonen, G Biberfeld, J M Middeldorp, P Biberfeld, I Ernberg

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Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.

Original languageEnglish
Pages (from-to)609-15
Number of pages7
JournalInternational journal of cancer
Issue number4
Publication statusPublished - 21 Oct 1993


  • Animals
  • Antigens, CD
  • Antigens, Nuclear
  • Antigens, Viral
  • Blotting, Western
  • Cross Reactions
  • DNA-Binding Proteins
  • Epstein-Barr Virus Nuclear Antigens
  • Herpesvirus 4, Human
  • Immunohistochemistry
  • Journal Article
  • Lymphoma, B-Cell
  • Macaca fascicularis
  • Nuclear Proteins
  • Research Support, Non-U.S. Gov't
  • Simian Immunodeficiency Virus

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