Abstract
Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.
| Original language | English |
|---|---|
| Pages (from-to) | 609-15 |
| Number of pages | 7 |
| Journal | International journal of cancer |
| Volume | 55 |
| Issue number | 4 |
| Publication status | Published - 21 Oct 1993 |
Keywords
- Animals
- Antigens, CD
- Antigens, Nuclear
- Antigens, Viral
- Blotting, Western
- Cross Reactions
- DNA-Binding Proteins
- Epstein-Barr Virus Nuclear Antigens
- Herpesvirus 4, Human
- Immunohistochemistry
- Journal Article
- Lymphoma, B-Cell
- Macaca fascicularis
- Nuclear Proteins
- Research Support, Non-U.S. Gov't
- Simian Immunodeficiency Virus