TY - JOUR
T1 - Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts
AU - Hansen, Marten
AU - Varga, Eszter
AU - Wüst, Tatjana
AU - Mellink, Clemens
AU - van der Kevie-Kersemaekers, Anne-Marie
AU - von Lindern, Marieke
AU - van den Akker, Emile
PY - 2017
Y1 - 2017
N2 - Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34(+)/CD41(+) megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM selfsilencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germlayers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for othermegakaryoid origin iPSC and could be applied for epigenetic based research. (c) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
AB - Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34(+)/CD41(+) megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM selfsilencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germlayers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for othermegakaryoid origin iPSC and could be applied for epigenetic based research. (c) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
U2 - https://doi.org/10.1016/j.scr.2017.10.002
DO - https://doi.org/10.1016/j.scr.2017.10.002
M3 - Article
C2 - 29055227
SN - 1873-5061
VL - 25
SP - 42
EP - 45
JO - Stem Cell Research
JF - Stem Cell Research
ER -