TY - JOUR
T1 - Genetic composition of replication competent clonal HIV-1 variants isolated from peripheral blood mononuclear cells (PBMC), HIV-1 proviral DNA from PBMC and HIV-1 RNA in serum in the course of HIV-1 infection
AU - Edo-Matas, Diana
AU - van Gils, Marit J.
AU - Bowles, Emma J.
AU - Navis, Marjon
AU - Rachinger, Andrea
AU - Boeser-Nunnink, Brigitte
AU - Stewart-Jones, Guillaume B.
AU - Kootstra, Neeltje A.
AU - Wout, Angélique B. van 't
AU - Schuitemaker, Hanneke
PY - 2010
Y1 - 2010
N2 - The HIV-1 quasispecies in peripheral blood mononuclear cells (PBMC) is considered to be a mix of actively replicating, latent, and archived viruses and may be genetically distinct from HIV-1 variants in plasma that are considered to be recently produced. Here we analyzed the genetic relationship between gp160 env sequences from replication competent clonal HIV-1 variants that were isolated from PBMC and from contemporaneous HIV-1 RNA in serum and HIV-1 proviral DNA in PBMC of four longitudinally studied therapy naive HIV-1 infected individuals. Replication competent clonal HIV-1 variants, HIV-1 RNA from serum, and HIV-1 proviral DNA from PBMC formed a single virus population at most time points analyzed. However, an under-representation in serum of HIV-1 sequences with predicted CXCR4 usage was sometimes observed implying that the analysis of viral sequences from different sources may provide a more complete assessment of the viral quasispecies in peripheral blood in vivo. (C) 2010 Elsevier Inc. All rights reserved
AB - The HIV-1 quasispecies in peripheral blood mononuclear cells (PBMC) is considered to be a mix of actively replicating, latent, and archived viruses and may be genetically distinct from HIV-1 variants in plasma that are considered to be recently produced. Here we analyzed the genetic relationship between gp160 env sequences from replication competent clonal HIV-1 variants that were isolated from PBMC and from contemporaneous HIV-1 RNA in serum and HIV-1 proviral DNA in PBMC of four longitudinally studied therapy naive HIV-1 infected individuals. Replication competent clonal HIV-1 variants, HIV-1 RNA from serum, and HIV-1 proviral DNA from PBMC formed a single virus population at most time points analyzed. However, an under-representation in serum of HIV-1 sequences with predicted CXCR4 usage was sometimes observed implying that the analysis of viral sequences from different sources may provide a more complete assessment of the viral quasispecies in peripheral blood in vivo. (C) 2010 Elsevier Inc. All rights reserved
U2 - https://doi.org/10.1016/j.virol.2010.06.029
DO - https://doi.org/10.1016/j.virol.2010.06.029
M3 - Article
C2 - 20638697
SN - 0042-6822
VL - 405
SP - 492
EP - 504
JO - Virology
JF - Virology
IS - 2
ER -