Glucocorticoid receptor ligand binding in monocytic cells using a microplate assay

J Jansen; B Uitdehaag; J W Koper; T K van Den Berg

doi:https://doi.org/10.1159/000028106

Glucocorticoid receptor ligand binding in monocytic cells using a microplate assay

J Jansen, B Uitdehaag, J W Koper, T K van Den Berg

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

Glucocorticoids have profound effects on macrophage function and are widely used as anti-inflammatory drugs. Glucocorticoids receptor (GR) ligand binding capacity is a major determinant of cellular glucocorticoid sensitivity. The number and affinity of GR can be measured in a whole cell binding assay using (3)H-dexamethasone. Here, we describe a rapid and simple microplate assay for GR measurement using the human promonocytic cell line THP-1.

Original language	English
Pages (from-to)	262-4
Number of pages	3
Journal	Pathobiology
Volume	67
Issue number	5-6
DOIs	https://doi.org/10.1159/000028106
Publication status	Published - 1999

Keywords

Cell Line
Dexamethasone/metabolism
Humans
Immunologic Techniques
Ligands
Monocytes/metabolism
Receptors, Glucocorticoid/metabolism

Access to Document

https://doi.org/10.1159/000028106

Cite this

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title = "Glucocorticoid receptor ligand binding in monocytic cells using a microplate assay",

abstract = "Glucocorticoids have profound effects on macrophage function and are widely used as anti-inflammatory drugs. Glucocorticoids receptor (GR) ligand binding capacity is a major determinant of cellular glucocorticoid sensitivity. The number and affinity of GR can be measured in a whole cell binding assay using (3)H-dexamethasone. Here, we describe a rapid and simple microplate assay for GR measurement using the human promonocytic cell line THP-1.",

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author = "J Jansen and B Uitdehaag and Koper, {J W} and {van Den Berg}, {T K}",

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AU - Jansen, J

AU - Uitdehaag, B

AU - Koper, J W

AU - van Den Berg, T K

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AB - Glucocorticoids have profound effects on macrophage function and are widely used as anti-inflammatory drugs. Glucocorticoids receptor (GR) ligand binding capacity is a major determinant of cellular glucocorticoid sensitivity. The number and affinity of GR can be measured in a whole cell binding assay using (3)H-dexamethasone. Here, we describe a rapid and simple microplate assay for GR measurement using the human promonocytic cell line THP-1.

KW - Cell Line

KW - Dexamethasone/metabolism

KW - Humans

KW - Immunologic Techniques

KW - Ligands

KW - Monocytes/metabolism

KW - Receptors, Glucocorticoid/metabolism

U2 - https://doi.org/10.1159/000028106

DO - https://doi.org/10.1159/000028106

M3 - Article

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SN - 1015-2008

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EP - 264

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