TY - JOUR
T1 - Human immature Langerhans cells restrict CXCR4-using HIV-1 transmission
AU - Sarrami-Forooshani, Ramin
AU - Mesman, Annelies W.
AU - van Teijlingen, Nienke H.
AU - Sprokholt, Joris K.
AU - van der Vlist, Michiel
AU - Ribeiro, Carla M. S.
AU - Geijtenbeek, Teunis B. H.
PY - 2014
Y1 - 2014
N2 - Sexual transmission is the main route of HIV-1 infection and the CCR5-using (R5) HIV-1 is predominantly transmitted, even though CXCR4-using (X4) HIV-1 is often abundant in chronic HIV-1 patients. The mechanisms underlying this tropism selection are unclear. Mucosal Langerhans cells (LCs) are the first immune cells to encounter HIV-1 and here we investigated the role of LCs in selection of R5 HIV-1 using an ex vivo epidermal and vaginal transmission models. Immature LCs were productively infected by X4 as well as R5 HIV-1. However, only R5 but not X4 viruses were selectively transmitted by immature LCs to T cells. Transmission of HIV-1 was depended on de novo production of HIV-1 in LCs, since it could be inhibited by CCR5 fusion inhibitors as well as reverse transcription inhibitors. Notably, the activation state of LCs affected the restriction in X4 HIV-1 transmission; immune activation by TNF facilitated transmission of X4 as well as R5 HIV-1. These data suggest that LCs play a crucial role in R5 selection and that immature LCs effectively restrict X4 at the level of transmission
AB - Sexual transmission is the main route of HIV-1 infection and the CCR5-using (R5) HIV-1 is predominantly transmitted, even though CXCR4-using (X4) HIV-1 is often abundant in chronic HIV-1 patients. The mechanisms underlying this tropism selection are unclear. Mucosal Langerhans cells (LCs) are the first immune cells to encounter HIV-1 and here we investigated the role of LCs in selection of R5 HIV-1 using an ex vivo epidermal and vaginal transmission models. Immature LCs were productively infected by X4 as well as R5 HIV-1. However, only R5 but not X4 viruses were selectively transmitted by immature LCs to T cells. Transmission of HIV-1 was depended on de novo production of HIV-1 in LCs, since it could be inhibited by CCR5 fusion inhibitors as well as reverse transcription inhibitors. Notably, the activation state of LCs affected the restriction in X4 HIV-1 transmission; immune activation by TNF facilitated transmission of X4 as well as R5 HIV-1. These data suggest that LCs play a crucial role in R5 selection and that immature LCs effectively restrict X4 at the level of transmission
U2 - https://doi.org/10.1186/1742-4690-11-52
DO - https://doi.org/10.1186/1742-4690-11-52
M3 - Article
C2 - 24990163
SN - 1742-4690
VL - 11
SP - 52
JO - Retrovirology
JF - Retrovirology
IS - 1
ER -