TY - JOUR
T1 - Identification and functional characterization of sRNAs in Neisseria meningitidis
AU - Pannekoek, Yvonne
AU - van der Ende, Arie
PY - 2012
Y1 - 2012
N2 - A riboregulated network, in which small RNAs (sRNAs) regulate the stability and thus translation of transcripts (mRNA), has only recently been discovered in prokaryotes. Yet, during the last 5 years, hundreds of sRNAs have been identified in various bacterial species by using a wide variety of both computational and experimental approaches. The majority of the sRNAs interact with the 5'-untranslated region (UTR) of target mRNAs, thereby influencing the stability of target mRNAs, or by either suppressing or upregulating the ribosome entry to the mRNAs influencing translation. Here, we describe experimental approaches successfully used in our laboratory to identify and functionally characterize sRNAs in vivo in our model micro-organism Neisseria meningitidis
AB - A riboregulated network, in which small RNAs (sRNAs) regulate the stability and thus translation of transcripts (mRNA), has only recently been discovered in prokaryotes. Yet, during the last 5 years, hundreds of sRNAs have been identified in various bacterial species by using a wide variety of both computational and experimental approaches. The majority of the sRNAs interact with the 5'-untranslated region (UTR) of target mRNAs, thereby influencing the stability of target mRNAs, or by either suppressing or upregulating the ribosome entry to the mRNAs influencing translation. Here, we describe experimental approaches successfully used in our laboratory to identify and functionally characterize sRNAs in vivo in our model micro-organism Neisseria meningitidis
U2 - https://doi.org/10.1007/978-1-61779-346-2_5
DO - https://doi.org/10.1007/978-1-61779-346-2_5
M3 - Article
C2 - 21993640
SN - 1064-3745
VL - 799
SP - 73
EP - 89
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -