Improved diagnostic stratification of digitised Barrett's oesophagus biopsies by p53 immunohistochemical staining

Myrtle J van der Wel, Lucas C Duits, Roos E Pouw, Cornelis A Seldenrijk, G J A Offerhaus, Mike Visser, Fiebo J Ten Kate, Katharina Biermann, Lodewijk A A Brosens, Michael Doukas, Clement Huysentruyt, Arend Karrenbeld, Gursah Kats-Ugurlu, Jaap S van der Laan, G Ineke van Lijnschoten, Freek C P Moll, Ariadne H A G Ooms, Hans van der Valk, Jan G Tijssen, Jacques J BergmanSybren L Meijer

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27 Citations (Scopus)


AIMS: Interobserver agreement for dysplasia in Barrett's oesophagus (BO) is low, and guidelines advise expert review of dysplastic cases. The aim of this study was to assess the added value of p53 immunohistochemistry (IHC) for the homogeneity within a group of dedicated gastrointestinal (GI) pathologists.

METHODS AND RESULTS: Sixty-single haematoxylin and eosin (HE) slide referral BO cases [20 low-grade dysplasia (LGD); 20 high-grade dysplasia (HGD); and 20 non-dysplastic BO reference cases] were digitalised and independently assessed twice in random order by 10 dedicated GI pathologists. After a 'wash-out' period, cases were reassessed with the addition of a corresponding p53 IHC slide. Outcomes were: (i) proportion of 'indefinite for dysplasia' (IND) diagnoses; (ii) interobserver agreement; and (iii) diagnostic accuracy as compared with a consensus 'gold standard' diagnosis defined at an earlier stage by five core expert BO pathologists after their assessment of this case set. Addition of p53 IHC decreased the mean proportion of IND diagnoses from 10 of 60 to eight of 60 (P = 0.071). Mean interobserver agreement increased significantly from 0.45 to 0.57 (P = 0.0021). The mean diagnostic accuracy increased significantly from 72% to 82% (P = 0.0072) after p53 IHC addition.

CONCLUSION: Addition of p53 IHC significantly improves the histological assessment of BO biopsies, even within a group of dedicated GI pathologists. It decreases the proportion of IND diagnoses, and increases interobserver agreement and diagnostic accuracy. This justifies the use of accessory p53 IHC within our upcoming national digital review panel for BO biopsy cases.

Original languageEnglish
Pages (from-to)1015-1023
Number of pages9
Issue number6
Early online date2018
Publication statusPublished - May 2018


  • Barrett Esophagus/diagnosis
  • Biomarkers/analysis
  • Biopsy
  • Humans
  • Image Interpretation, Computer-Assisted/methods
  • Immunohistochemistry
  • Observer Variation
  • Tumor Suppressor Protein p53/analysis

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