Improved HIV-1 specific T-cell responses by short-interval DNA tattooing as compared to intramuscular immunization in non-human primates

Babs E. Verstrepen; Adriaan D. Bins; Christine S. Rollier; Petra Mooij; Gerrit Koopman; Neil C. Sheppard; Quentin Sattentau; Ralf Wagner; Hans Wolf; Ton N. M. Schumacher; Jonathan L. Heeney; John B. A. G. Haanen

doi:https://doi.org/10.1016/j.vaccine.2008.03.091

Improved HIV-1 specific T-cell responses by short-interval DNA tattooing as compared to intramuscular immunization in non-human primates

Babs E. Verstrepen, Adriaan D. Bins, Christine S. Rollier, Petra Mooij, Gerrit Koopman, Neil C. Sheppard, Quentin Sattentau, Ralf Wagner, Hans Wolf, Ton N. M. Schumacher, Jonathan L. Heeney, John B. A. G. Haanen

Research output: Contribution to journal › Article › Academic › peer-review

45 Citations (Scopus)

Abstract

The new intradermal DNA delivery technique, termed DNA tattooing might overcome the discrepancy between the encouraging immunogenicity results obtained with DNA vaccines in murine studies and the poor results obtained in non-human primates and humans, the so called "simian barrier". Here, we demonstrate a 10- to 100-fold increase in the magnitude of vaccine specific T-cell responses in peripheral blood from DNA tattooed rhesus macaques, as compared to T-cell responses in animals immunized via intramuscular (IM) route. A marked increase in the magnitude of the antigen specific T-cell responses as well as an increase in the number of animals responding to the immunogens was observed. These findings in non-human primates suggest that similar results may be observed in humans. Clinical trials are planned to validate tattooing as an optimal method of DNA vaccine delivery in humans

Original language	English
Pages (from-to)	3346-3351
Journal	Vaccine
Volume	26
Issue number	26
DOIs	https://doi.org/10.1016/j.vaccine.2008.03.091
Publication status	Published - 2008

Access to Document

https://doi.org/10.1016/j.vaccine.2008.03.091

Cite this

Verstrepen, B. E., Bins, A. D., Rollier, C. S., Mooij, P., Koopman, G., Sheppard, N. C., Sattentau, Q., Wagner, R., Wolf, H., Schumacher, T. N. M., Heeney, J. L., & Haanen, J. B. A. G. (2008). Improved HIV-1 specific T-cell responses by short-interval DNA tattooing as compared to intramuscular immunization in non-human primates. Vaccine, 26(26), 3346-3351. https://doi.org/10.1016/j.vaccine.2008.03.091

@article{fab8a3f39a114618b819142ff8810dd6,

title = "Improved HIV-1 specific T-cell responses by short-interval DNA tattooing as compared to intramuscular immunization in non-human primates",

abstract = "The new intradermal DNA delivery technique, termed DNA tattooing might overcome the discrepancy between the encouraging immunogenicity results obtained with DNA vaccines in murine studies and the poor results obtained in non-human primates and humans, the so called {"}simian barrier{"}. Here, we demonstrate a 10- to 100-fold increase in the magnitude of vaccine specific T-cell responses in peripheral blood from DNA tattooed rhesus macaques, as compared to T-cell responses in animals immunized via intramuscular (IM) route. A marked increase in the magnitude of the antigen specific T-cell responses as well as an increase in the number of animals responding to the immunogens was observed. These findings in non-human primates suggest that similar results may be observed in humans. Clinical trials are planned to validate tattooing as an optimal method of DNA vaccine delivery in humans",

author = "Verstrepen, {Babs E.} and Bins, {Adriaan D.} and Rollier, {Christine S.} and Petra Mooij and Gerrit Koopman and Sheppard, {Neil C.} and Quentin Sattentau and Ralf Wagner and Hans Wolf and Schumacher, {Ton N. M.} and Heeney, {Jonathan L.} and Haanen, {John B. A. G.}",

year = "2008",

doi = "https://doi.org/10.1016/j.vaccine.2008.03.091",

language = "English",

volume = "26",

pages = "3346--3351",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "26",

}

Verstrepen, BE, Bins, AD, Rollier, CS, Mooij, P, Koopman, G, Sheppard, NC, Sattentau, Q, Wagner, R, Wolf, H, Schumacher, TNM, Heeney, JL & Haanen, JBAG 2008, 'Improved HIV-1 specific T-cell responses by short-interval DNA tattooing as compared to intramuscular immunization in non-human primates', Vaccine, vol. 26, no. 26, pp. 3346-3351. https://doi.org/10.1016/j.vaccine.2008.03.091

TY - JOUR

T1 - Improved HIV-1 specific T-cell responses by short-interval DNA tattooing as compared to intramuscular immunization in non-human primates

AU - Verstrepen, Babs E.

AU - Bins, Adriaan D.

AU - Rollier, Christine S.

AU - Mooij, Petra

AU - Koopman, Gerrit

AU - Sheppard, Neil C.

AU - Sattentau, Quentin

AU - Wagner, Ralf

AU - Wolf, Hans

AU - Schumacher, Ton N. M.

AU - Heeney, Jonathan L.

AU - Haanen, John B. A. G.

PY - 2008

Y1 - 2008

N2 - The new intradermal DNA delivery technique, termed DNA tattooing might overcome the discrepancy between the encouraging immunogenicity results obtained with DNA vaccines in murine studies and the poor results obtained in non-human primates and humans, the so called "simian barrier". Here, we demonstrate a 10- to 100-fold increase in the magnitude of vaccine specific T-cell responses in peripheral blood from DNA tattooed rhesus macaques, as compared to T-cell responses in animals immunized via intramuscular (IM) route. A marked increase in the magnitude of the antigen specific T-cell responses as well as an increase in the number of animals responding to the immunogens was observed. These findings in non-human primates suggest that similar results may be observed in humans. Clinical trials are planned to validate tattooing as an optimal method of DNA vaccine delivery in humans

AB - The new intradermal DNA delivery technique, termed DNA tattooing might overcome the discrepancy between the encouraging immunogenicity results obtained with DNA vaccines in murine studies and the poor results obtained in non-human primates and humans, the so called "simian barrier". Here, we demonstrate a 10- to 100-fold increase in the magnitude of vaccine specific T-cell responses in peripheral blood from DNA tattooed rhesus macaques, as compared to T-cell responses in animals immunized via intramuscular (IM) route. A marked increase in the magnitude of the antigen specific T-cell responses as well as an increase in the number of animals responding to the immunogens was observed. These findings in non-human primates suggest that similar results may be observed in humans. Clinical trials are planned to validate tattooing as an optimal method of DNA vaccine delivery in humans

U2 - https://doi.org/10.1016/j.vaccine.2008.03.091

DO - https://doi.org/10.1016/j.vaccine.2008.03.091

M3 - Article

C2 - 18467010

SN - 0264-410X

VL - 26

SP - 3346

EP - 3351

JO - Vaccine

JF - Vaccine

IS - 26

ER -