@article{2e63cd7adbe04da1bf5af8ae4086ab0f,
title = "In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells",
abstract = "Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-term 3D co-culture system of human intestinal CD4+ T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).",
keywords = "Cell culture, Immunology, Organoids",
author = "Schreurs, {Ren{\'e}e R. C. E.} and Baumdick, {Martin E.} and Agata Drewniak and Bunders, {Madeleine J.}",
note = "Funding Information: The authors would like to thank Dr. Weijer, Dr. Voordouw, Mrs. Siteur-van Rijnstra, and the Bloemenhove Clinic (Heemstede, the Netherlands) for collecting tissues; Adrian Sagebiel, Deborah Sandfort, and Biorender.com for design of illustrations; and Anusca Rader for assistance in acquiring organoid images. This work was financially supported by the Dutch Digestive Fund (MLDS Project CDG 15-02), the Deutsche Forschungsgemeinschaft (DFG; SFB841), the Stichting Steun Emma (WAR008-2015-03-002), and the Daisy Hu?t R?ell Foundation. R.R.C.E.S. M.E.B. A.D. and M.J.B. designed and performed the experiments. R.R.C.E.S. M.E.B. and A.D. collected samples and analyzed the data. R.R.C.E.S. M.E.B. and M.J.B. wrote the protocol. M.J.B. supervised the study. The authors declare no competing interests. Funding Information: The authors would like to thank Dr. Weijer, Dr. Voordouw, Mrs. Siteur-van Rijnstra, and the Bloemenhove Clinic (Heemstede, the Netherlands) for collecting tissues; Adrian Sagebiel, Deborah Sandfort, and Biorender.com for design of illustrations; and Anusca Rader for assistance in acquiring organoid images. This work was financially supported by the Dutch Digestive Fund ( MLDS Project CDG 15-02 ), the Deutsche Forschungsgemeinschaft ( DFG ; SFB841 ), the Stichting Steun Emma ( WAR008-2015-03-002 ), and the Daisy Hu{\"e}t R{\"o}ell Foundation . Publisher Copyright: {\textcopyright} 2021 The Author(s) Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = jun,
day = "18",
doi = "https://doi.org/10.1016/j.xpro.2021.100519",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}