Innate cell markers that predict anti-HIV neutralizing antibody titers in vaccinated macaques

Matthieu van Tilbeurgh, Pauline Maisonnasse, Jean-Louis Palgen, Monica Tolazzi, Yoann Aldon, Nathalie Dereuddre-Bosquet, Mariangela Cavarelli, Anne-Sophie Beignon, Ernesto Marcos-Lopez, Anne-Sophie Gallouet, Emmanuel Gilson, Gabriel Ozorowski, Andrew B. Ward, Ilja Bontjer, Paul F. McKay, Robin J. Shattock, Gabriella Scarlatti, Rogier W. Sanders, Roger le Grand

Research output: Contribution to journalArticleAcademicpeer-review


Given the time and resources invested in clinical trials, innovative prediction methods are needed to decrease late-stage failure in vaccine development. We identify combinations of early innate responses that predict neutralizing antibody (nAb) responses induced in HIV-Env SOSIP immunized cynomolgus macaques using various routes of vaccine injection and adjuvants. We analyze blood myeloid cells before and 24 h after each immunization by mass cytometry using a three-step clustering, and we discriminate unique vaccine signatures based on HLA-DR, CD39, CD86, CD11b, CD45, CD64, CD14, CD32, CD11c, CD123, CD4, CD16, and CADM1 surface expression. Various combinations of these markers characterize cell families positively associated with nAb production, whereas CADM1-expressing cells are negatively associated (p < 0.05). Our results demonstrate that monitoring immune signatures during early vaccine development could assist in identifying biomarkers that predict vaccine immunogenicity.

Original languageEnglish
Article number100751
JournalCell Reports Medicine
Issue number10
Publication statusPublished - 18 Oct 2022


  • HIV vaccine
  • cynomolgus macaques
  • innate cells
  • mass cytometry
  • predictive model
  • system vaccinology

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