Interleukin-18 impairs the pulmonary host response to Pseudomonas aeruginosa

Marc J. Schultz; Sylvia Knapp; Sandrine Florquin; Jennie Pater; Kiyoshi Takeda; Shizuo Akira; Tom van der Poll

doi:https://doi.org/10.1128/IAI.71.4.1630-1634.2003

Interleukin-18 impairs the pulmonary host response to Pseudomonas aeruginosa

Marc J. Schultz, Sylvia Knapp, Sandrine Florquin, Jennie Pater, Kiyoshi Takeda, Shizuo Akira, Tom van der Poll

Research output: Contribution to journal › Article › Academic › peer-review

60 Citations (Scopus)

Abstract

Interleukin-18 (IL-18) is a potent cytokine with many different proinflammatory activities. To study the role of IL-18 in the pathogenesis of Pseudomonas pneumonia, IL-18-deficient (IL-18(-/-)) and wild-type mice were intranasally inoculated with Pseudomonas aeruginosa. IL-18 deficiency was associated with reduced outgrowth of Pseudomonas in the lungs and diminished dissemination of the infection. In addition, pulmonary inflammation (histopathology) and levels of tumor necrosis factor alpha, IL-6, and macrophage inflammatory protein-2 in lungs and plasma were lower in IL-18(-/-) mice. Consistent with results obtained for IL-18(-/-) mice, treatment of wild-type mice with a neutralizing IL-18 binding protein-immunoglobulin G Fe fusion construct also attenuated outgrowth of Pseudomonas compared with that for mice treated with a control protein. These results demonstrate that the presence of endogenous IL-18 activity facilitates inflammatory responses in the lung during Pseudomonas pneumonia, concurrently impairing bacterial clearance

Original language	English
Pages (from-to)	1630-1634
Journal	Infection and immunity
Volume	71
Issue number	4
DOIs	https://doi.org/10.1128/IAI.71.4.1630-1634.2003
Publication status	Published - 2003

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https://doi.org/10.1128/IAI.71.4.1630-1634.2003

Cite this

@article{65f68a7bafda49a68200e25e738906bf,

title = "Interleukin-18 impairs the pulmonary host response to Pseudomonas aeruginosa",

abstract = "Interleukin-18 (IL-18) is a potent cytokine with many different proinflammatory activities. To study the role of IL-18 in the pathogenesis of Pseudomonas pneumonia, IL-18-deficient (IL-18(-/-)) and wild-type mice were intranasally inoculated with Pseudomonas aeruginosa. IL-18 deficiency was associated with reduced outgrowth of Pseudomonas in the lungs and diminished dissemination of the infection. In addition, pulmonary inflammation (histopathology) and levels of tumor necrosis factor alpha, IL-6, and macrophage inflammatory protein-2 in lungs and plasma were lower in IL-18(-/-) mice. Consistent with results obtained for IL-18(-/-) mice, treatment of wild-type mice with a neutralizing IL-18 binding protein-immunoglobulin G Fe fusion construct also attenuated outgrowth of Pseudomonas compared with that for mice treated with a control protein. These results demonstrate that the presence of endogenous IL-18 activity facilitates inflammatory responses in the lung during Pseudomonas pneumonia, concurrently impairing bacterial clearance",

author = "Schultz, {Marc J.} and Sylvia Knapp and Sandrine Florquin and Jennie Pater and Kiyoshi Takeda and Shizuo Akira and {van der Poll}, Tom",

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language = "English",

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journal = "Infection and immunity",

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publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Interleukin-18 impairs the pulmonary host response to Pseudomonas aeruginosa

AU - Schultz, Marc J.

AU - Knapp, Sylvia

AU - Florquin, Sandrine

AU - Pater, Jennie

AU - Takeda, Kiyoshi

AU - Akira, Shizuo

AU - van der Poll, Tom

PY - 2003

Y1 - 2003

N2 - Interleukin-18 (IL-18) is a potent cytokine with many different proinflammatory activities. To study the role of IL-18 in the pathogenesis of Pseudomonas pneumonia, IL-18-deficient (IL-18(-/-)) and wild-type mice were intranasally inoculated with Pseudomonas aeruginosa. IL-18 deficiency was associated with reduced outgrowth of Pseudomonas in the lungs and diminished dissemination of the infection. In addition, pulmonary inflammation (histopathology) and levels of tumor necrosis factor alpha, IL-6, and macrophage inflammatory protein-2 in lungs and plasma were lower in IL-18(-/-) mice. Consistent with results obtained for IL-18(-/-) mice, treatment of wild-type mice with a neutralizing IL-18 binding protein-immunoglobulin G Fe fusion construct also attenuated outgrowth of Pseudomonas compared with that for mice treated with a control protein. These results demonstrate that the presence of endogenous IL-18 activity facilitates inflammatory responses in the lung during Pseudomonas pneumonia, concurrently impairing bacterial clearance

AB - Interleukin-18 (IL-18) is a potent cytokine with many different proinflammatory activities. To study the role of IL-18 in the pathogenesis of Pseudomonas pneumonia, IL-18-deficient (IL-18(-/-)) and wild-type mice were intranasally inoculated with Pseudomonas aeruginosa. IL-18 deficiency was associated with reduced outgrowth of Pseudomonas in the lungs and diminished dissemination of the infection. In addition, pulmonary inflammation (histopathology) and levels of tumor necrosis factor alpha, IL-6, and macrophage inflammatory protein-2 in lungs and plasma were lower in IL-18(-/-) mice. Consistent with results obtained for IL-18(-/-) mice, treatment of wild-type mice with a neutralizing IL-18 binding protein-immunoglobulin G Fe fusion construct also attenuated outgrowth of Pseudomonas compared with that for mice treated with a control protein. These results demonstrate that the presence of endogenous IL-18 activity facilitates inflammatory responses in the lung during Pseudomonas pneumonia, concurrently impairing bacterial clearance

U2 - https://doi.org/10.1128/IAI.71.4.1630-1634.2003

DO - https://doi.org/10.1128/IAI.71.4.1630-1634.2003

M3 - Article

C2 - 12654774

SN - 0019-9567

VL - 71

SP - 1630

EP - 1634

JO - Infection and immunity

JF - Infection and immunity

IS - 4

ER -