TY - JOUR
T1 - Localized H3K36 methylation states define histone H4K16 acetylation during transcriptional elongation in Drosophila
AU - Bell, Oliver
AU - Wirbelauer, Christiane
AU - Hild, Marc
AU - Scharf, Annette N. D.
AU - Schwaiger, Michaela
AU - MacAlpine, David M.
AU - Zilbermann, Frédéric
AU - van Leeuwen, Fred
AU - Bell, Stephen P.
AU - Imhof, Axel
AU - Garza, Dan
AU - Peters, Antoine H. F. M.
AU - Schübeler, Dirk
PY - 2007
Y1 - 2007
N2 - Post-translational modifications of histones are involved in transcript initiation and elongation. Methylation of lysine 36 of histone H3 (H3K36me) resides promoter distal at transcribed regions in Saccharomyces cerevisiae and is thought to prevent spurious initiation through recruitment of histone-deacetylase activity. Here, we report surprising complexity in distribution, regulation and readout of H3K36me in Drosophila involving two histone methyltransferases (HMTases). Dimethylation of H3K36 peaks adjacent to promoters and requires dMes-4, whereas trimethylation accumulates toward the 3′ end of genes and relies on dHypb. Reduction of H3K36me3 is lethal in Drosophila larvae and leads to elevated levels of acetylation, specifically at lysine 16 of histone H4 (H4K16ac). In contrast, reduction of both di- and trimethylation decreases lysine 16 acetylation. Thus di- and trimethylation of H3K36 have opposite effects on H4K16 acetylation, which we propose enable dynamic changes in chromatin compaction during transcript elongation. ©2007 European Molecular Biology Organization.
AB - Post-translational modifications of histones are involved in transcript initiation and elongation. Methylation of lysine 36 of histone H3 (H3K36me) resides promoter distal at transcribed regions in Saccharomyces cerevisiae and is thought to prevent spurious initiation through recruitment of histone-deacetylase activity. Here, we report surprising complexity in distribution, regulation and readout of H3K36me in Drosophila involving two histone methyltransferases (HMTases). Dimethylation of H3K36 peaks adjacent to promoters and requires dMes-4, whereas trimethylation accumulates toward the 3′ end of genes and relies on dHypb. Reduction of H3K36me3 is lethal in Drosophila larvae and leads to elevated levels of acetylation, specifically at lysine 16 of histone H4 (H4K16ac). In contrast, reduction of both di- and trimethylation decreases lysine 16 acetylation. Thus di- and trimethylation of H3K36 have opposite effects on H4K16 acetylation, which we propose enable dynamic changes in chromatin compaction during transcript elongation. ©2007 European Molecular Biology Organization.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=37149047905&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/18007591
U2 - https://doi.org/10.1038/sj.emboj.7601926
DO - https://doi.org/10.1038/sj.emboj.7601926
M3 - Article
C2 - 18007591
SN - 0261-4189
VL - 26
SP - 4974
EP - 4984
JO - EMBO Journal
JF - EMBO Journal
IS - 24
ER -