Lymphocyte adhesion to brain capillary endothelial cells in vitro

Helga E. de Vries, Anne C.E. Moor, Margret C.M. Blom-Roosemalen, Albertus G. de Boer, Douwe D. Breimer, Theo J.C. van Berkel, Johan Kuiper

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The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1β (rhIL-1β), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJournal of Neuroimmunology
Issue number1
Publication statusPublished - Jun 1994


  • Blood-brain barrier
  • Cerebral endothelial cells
  • Interleukin
  • Lipopolysaccharide

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