TY - JOUR
T1 - Lymphocyte adhesion to brain capillary endothelial cells in vitro
AU - de Vries, Helga E.
AU - Moor, Anne C.E.
AU - Blom-Roosemalen, Margret C.M.
AU - de Boer, Albertus G.
AU - Breimer, Douwe D.
AU - van Berkel, Theo J.C.
AU - Kuiper, Johan
PY - 1994/6
Y1 - 1994/6
N2 - The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1β (rhIL-1β), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier.
AB - The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1β (rhIL-1β), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier.
KW - Blood-brain barrier
KW - Cerebral endothelial cells
KW - Interleukin
KW - Lipopolysaccharide
UR - http://www.scopus.com/inward/record.url?scp=0028223718&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/0165-5728(94)90155-4
DO - https://doi.org/10.1016/0165-5728(94)90155-4
M3 - Article
C2 - 7911476
SN - 0165-5728
VL - 52
SP - 1
EP - 8
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1
ER -