New oligopeptide immunoglobulin G test for human parvovirus B19 antibodies

T F Schwarz, S Modrow, B Hottenträger, B Höflacher, G Jäger, W Scharti, R Sumazakl, H Wolf, J Middeldorp, M Roggendorf

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Abstract

A new, highly sensitive and specific enzyme immunoassay using oligopeptides as antigen (enzyme-linked immunosorbent assay [ELISA] B19-OP) for detecting parvovirus B19-specific immunoglobulin G (IgG) was established. As antigens, B19-specific oligopeptides of 24 and 30 kDa derived from a 196-kDa fusion protein of beta-galactosidase and viral capsid protein (VPI) of B19 after CNBr cleavage and separation by high-pressure liquid chromatography were used. Of 139 serum specimens tested in parallel for anti-B19 IgG by standard ELISA using B19 particles as antigen and by ELISA B19-OP, 73 (52.5%) were positive and 63 (45.3%) were negative in both tests, and 3 (2.2%) were negative by standard ELISA but positive by ELISA B19-OP and by immunoblot. By using ELISA B19-OP, it was possible to detect anti-B19 IgG in an asymptomatic blood donor 4 weeks after acute infection, and anti-B19 IgG titers of 10(-5) could be measured in convalescent-phase sera.

Original languageEnglish
Pages (from-to)431-5
Number of pages5
JournalJournal of Clinical Microbiology
Volume29
Issue number3
Publication statusPublished - Mar 1991

Keywords

  • Antibodies, Viral
  • Antigens, Viral
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoglobulin G
  • Journal Article
  • Oligopeptides
  • Parvoviridae
  • Parvoviridae Infections
  • Research Support, Non-U.S. Gov't
  • Sensitivity and Specificity

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