Nuclear recruitment assay as a tool to validate transcription factor interactions in Mammalian cells

C. J. J. Boogerd; V. M. Christoffels; P. Barnett

doi:https://doi.org/10.1007/978-1-62703-284-1_19

Nuclear recruitment assay as a tool to validate transcription factor interactions in Mammalian cells

C. J. J. Boogerd, V. M. Christoffels, P. Barnett

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Identification and verification of novel transcription factor interactions is an inherent step in the discovery of molecular mechanisms driving gene transcription and regulation. Co-immunoprecipitation and GST-pull down are often key techniques in the verification process. Despite wide applicability, their use may sometimes be restricted. We provide a detailed protocol for an intracellular immunofluorescence technique that may be used as an alternative or complimentary study for transcription factor interaction verification

Original language	English
Pages (from-to)	243-248
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	977
DOIs	https://doi.org/10.1007/978-1-62703-284-1_19
Publication status	Published - 2013

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https://doi.org/10.1007/978-1-62703-284-1_19

Cite this

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title = "Nuclear recruitment assay as a tool to validate transcription factor interactions in Mammalian cells",

abstract = "Identification and verification of novel transcription factor interactions is an inherent step in the discovery of molecular mechanisms driving gene transcription and regulation. Co-immunoprecipitation and GST-pull down are often key techniques in the verification process. Despite wide applicability, their use may sometimes be restricted. We provide a detailed protocol for an intracellular immunofluorescence technique that may be used as an alternative or complimentary study for transcription factor interaction verification",

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