Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors

Lin Luo; Mateusz C. Ambrozkiewicz; Fritz Benseler; Cui Chen; Emilie Dumontier; Susanne Falkner; Elisabetta Furlanis; Andrea M. Gomez; Naosuke Hoshina; Wei Hsiang Huang; Mary Anne Hutchison; Yu Itoh-Maruoka; Laura A. Lavery; Wei Li; Tomohiko Maruo; Junko Motohashi; Emily Ling Lin Pai; Kenneth A. Pelkey; Ariane Pereira; Thomas Philips; Jennifer L. Sinclair; Jeff A. Stogsdill; Lisa Traunmüller; Jiexin Wang; Joke Wortel; Wenjia You; Nashat Abumaria; Kevin T. Beier; Nils Brose; Harold A. Burgess; Constance L. Cepko; Jean François Cloutier; Cagla Eroglu; Sandra Goebbels; Pascal S. Kaeser; Jeremy N. Kay; Wei Lu; Liqun Luo; Kenji Mandai; Chris J. McBain; Klaus Armin Nave; Marco A.M. Prado; Vania F. Prado; Jeffrey Rothstein; John L.R. Rubenstein; Gesine Saher; Kenji Sakimura; Joshua R. Sanes; Peter Scheiffele; Yoshimi Takai; Hisashi Umemori; Matthijs Verhage; Michisuke Yuzaki; Huda Yahya Zoghbi; Hiroshi Kawabe; Ann Marie Craig

doi:https://doi.org/10.1016/j.neuron.2020.01.008

Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors

Lin Luo, Mateusz C. Ambrozkiewicz, Fritz Benseler, Cui Chen, Emilie Dumontier, Susanne Falkner, Elisabetta Furlanis, Andrea M. Gomez, Naosuke Hoshina, Wei Hsiang Huang, Mary Anne Hutchison, Yu Itoh-Maruoka, Laura A. Lavery, Wei Li, Tomohiko Maruo, Junko Motohashi, Emily Ling Lin Pai, Kenneth A. Pelkey, Ariane Pereira, Thomas PhilipsJennifer L. Sinclair, Jeff A. Stogsdill, Lisa Traunmüller, Jiexin Wang, Joke Wortel, Wenjia You, Nashat Abumaria, Kevin T. Beier, Nils Brose, Harold A. Burgess, Constance L. Cepko, Jean François Cloutier, Cagla Eroglu, Sandra Goebbels, Pascal S. Kaeser, Jeremy N. Kay, Wei Lu, Liqun Luo, Kenji Mandai, Chris J. McBain, Klaus Armin Nave, Marco A.M. Prado, Vania F. Prado, Jeffrey Rothstein, John L.R. Rubenstein, Gesine Saher, Kenji Sakimura, Joshua R. Sanes, Peter Scheiffele, Yoshimi Takai, Hisashi Umemori, Matthijs Verhage, Michisuke Yuzaki, Huda Yahya Zoghbi, Hiroshi Kawabe, Ann Marie Craig

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Abstract

Luo et al. report variable rates of germline recombination in commonly used mouse Cre driver lines, influenced by sex of Cre-carrying parents and target loci. Guidelines are provided to optimize cell-type-specific recombination in genetically targeted organisms expressing site-specific recombinases.

Original language	English
Pages (from-to)	37-65.e5
Number of pages	35
Journal	Neuron
Volume	106
Issue number	1
Early online date	5 Feb 2020
DOIs	https://doi.org/10.1016/j.neuron.2020.01.008
Publication status	Published - 8 Apr 2020

Keywords

Cre-lox
conditional gene targeting
conditional knockin
conditional knockout
conditional reporter
germline recombination
molecular genetics
mosaic recombination
parental sex bias
site-specific recombinase

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https://doi.org/10.1016/j.neuron.2020.01.008

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Luo, L., Ambrozkiewicz, M. C., Benseler, F., Chen, C., Dumontier, E., Falkner, S., Furlanis, E., Gomez, A. M., Hoshina, N., Huang, W. H., Hutchison, M. A., Itoh-Maruoka, Y., Lavery, L. A., Li, W., Maruo, T., Motohashi, J., Pai, E. L. L., Pelkey, K. A., Pereira, A., ... Craig, A. M. (2020). Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors. Neuron, 106(1), 37-65.e5. https://doi.org/10.1016/j.neuron.2020.01.008

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abstract = "Luo et al. report variable rates of germline recombination in commonly used mouse Cre driver lines, influenced by sex of Cre-carrying parents and target loci. Guidelines are provided to optimize cell-type-specific recombination in genetically targeted organisms expressing site-specific recombinases.",

keywords = "Cre-lox, conditional gene targeting, conditional knockin, conditional knockout, conditional reporter, germline recombination, molecular genetics, mosaic recombination, parental sex bias, site-specific recombinase",

author = "Lin Luo and Ambrozkiewicz, {Mateusz C.} and Fritz Benseler and Cui Chen and Emilie Dumontier and Susanne Falkner and Elisabetta Furlanis and Gomez, {Andrea M.} and Naosuke Hoshina and Huang, {Wei Hsiang} and Hutchison, {Mary Anne} and Yu Itoh-Maruoka and Lavery, {Laura A.} and Wei Li and Tomohiko Maruo and Junko Motohashi and Pai, {Emily Ling Lin} and Pelkey, {Kenneth A.} and Ariane Pereira and Thomas Philips and Sinclair, {Jennifer L.} and Stogsdill, {Jeff A.} and Lisa Traunm{\"u}ller and Jiexin Wang and Joke Wortel and Wenjia You and Nashat Abumaria and Beier, {Kevin T.} and Nils Brose and Burgess, {Harold A.} and Cepko, {Constance L.} and Cloutier, {Jean Fran{\c c}ois} and Cagla Eroglu and Sandra Goebbels and Kaeser, {Pascal S.} and Kay, {Jeremy N.} and Wei Lu and Liqun Luo and Kenji Mandai and McBain, {Chris J.} and Nave, {Klaus Armin} and Prado, {Marco A.M.} and Prado, {Vania F.} and Jeffrey Rothstein and Rubenstein, {John L.R.} and Gesine Saher and Kenji Sakimura and Sanes, {Joshua R.} and Peter Scheiffele and Yoshimi Takai and Hisashi Umemori and Matthijs Verhage and Michisuke Yuzaki and Zoghbi, {Huda Yahya} and Hiroshi Kawabe and Craig, {Ann Marie}",

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Luo, L, Ambrozkiewicz, MC, Benseler, F, Chen, C, Dumontier, E, Falkner, S, Furlanis, E, Gomez, AM, Hoshina, N, Huang, WH, Hutchison, MA, Itoh-Maruoka, Y, Lavery, LA, Li, W, Maruo, T, Motohashi, J, Pai, ELL, Pelkey, KA, Pereira, A, Philips, T, Sinclair, JL, Stogsdill, JA, Traunmüller, L, Wang, J, Wortel, J, You, W, Abumaria, N, Beier, KT, Brose, N, Burgess, HA, Cepko, CL, Cloutier, JF, Eroglu, C, Goebbels, S, Kaeser, PS, Kay, JN, Lu, W, Luo, L, Mandai, K, McBain, CJ, Nave, KA, Prado, MAM, Prado, VF, Rothstein, J, Rubenstein, JLR, Saher, G, Sakimura, K, Sanes, JR, Scheiffele, P, Takai, Y, Umemori, H, Verhage, M, Yuzaki, M, Zoghbi, HY, Kawabe, H & Craig, AM 2020, 'Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors', Neuron, vol. 106, no. 1, pp. 37-65.e5. https://doi.org/10.1016/j.neuron.2020.01.008

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T2 - Prevalence of Germline Recombination and Influencing Factors

AU - Luo, Lin

AU - Ambrozkiewicz, Mateusz C.

AU - Benseler, Fritz

AU - Chen, Cui

AU - Dumontier, Emilie

AU - Falkner, Susanne

AU - Furlanis, Elisabetta

AU - Gomez, Andrea M.

AU - Hoshina, Naosuke

AU - Huang, Wei Hsiang

AU - Hutchison, Mary Anne

AU - Itoh-Maruoka, Yu

AU - Lavery, Laura A.

AU - Li, Wei

AU - Maruo, Tomohiko

AU - Motohashi, Junko

AU - Pai, Emily Ling Lin

AU - Pelkey, Kenneth A.

AU - Pereira, Ariane

AU - Philips, Thomas

AU - Sinclair, Jennifer L.

AU - Stogsdill, Jeff A.

AU - Traunmüller, Lisa

AU - Wang, Jiexin

AU - Wortel, Joke

AU - You, Wenjia

AU - Abumaria, Nashat

AU - Beier, Kevin T.

AU - Brose, Nils

AU - Burgess, Harold A.

AU - Cepko, Constance L.

AU - Cloutier, Jean François

AU - Eroglu, Cagla

AU - Goebbels, Sandra

AU - Kaeser, Pascal S.

AU - Kay, Jeremy N.

AU - Lu, Wei

AU - Luo, Liqun

AU - Mandai, Kenji

AU - McBain, Chris J.

AU - Nave, Klaus Armin

AU - Prado, Marco A.M.

AU - Prado, Vania F.

AU - Rothstein, Jeffrey

AU - Rubenstein, John L.R.

AU - Saher, Gesine

AU - Sakimura, Kenji

AU - Sanes, Joshua R.

AU - Scheiffele, Peter

AU - Takai, Yoshimi

AU - Umemori, Hisashi

AU - Verhage, Matthijs

AU - Yuzaki, Michisuke

AU - Zoghbi, Huda Yahya

AU - Kawabe, Hiroshi

AU - Craig, Ann Marie

PY - 2020/4/8

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KW - conditional gene targeting

KW - conditional knockin

KW - conditional knockout

KW - conditional reporter

KW - germline recombination

KW - molecular genetics

KW - mosaic recombination

KW - parental sex bias

KW - site-specific recombinase

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SN - 0896-6273

VL - 106

SP - 37-65.e5

JO - Neuron

JF - Neuron

IS - 1

ER -

Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors

Abstract

Keywords

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