TY - JOUR
T1 - Perfluorohexane-loaded macrophages as a novel ultrasound contrast agent: a feasibility study
AU - Kornmann, Liselotte M.
AU - Curfs, Daniëlle M. J.
AU - Hermeling, Evelien
AU - van der Made, Ingeborg
AU - de Winther, Menno P. J.
AU - Reneman, Robert S.
AU - Reesink, Koen D.
AU - Hoeks, Arnold P. G.
PY - 2008
Y1 - 2008
N2 - We investigated in vitro the potential of macrophages to act as targeted vehicle for ultrasound molecular imaging. Murine bone marrow-derived macrophages (BMM), incubated for 3 h with different concentrations of perfluorohexane (PFH) emulsions, were monitored by microscopy, flow cytometry, and ultrasound. Effects of PFH loading on BMM adhesion molecule (PSGL-1, VLA-4, Mac-1, LFA-1) expression were analyzed by flow cytometry. Static adhesion of PFH loaded BMM to unstimulated and TNF-alpha stimulated b.End5 endothelial cells was assessed by microscopy. Incubation of BMM with PFH emulsions resulted in dose-dependent uptake and increased echogenicity (max. 17 dB). Flow cytometry analyses revealed no down-regulation related to PFH loading of BMM adhesion molecule expression. Endothelial adhesion remained functional, even after 24 h, although PFH loading dose-dependently attenuated static adhesion. PFH loaded BMM may potentially serve as ultrasound contrast agent for noninvasive detection of atherogenic hotspots in arteries
AB - We investigated in vitro the potential of macrophages to act as targeted vehicle for ultrasound molecular imaging. Murine bone marrow-derived macrophages (BMM), incubated for 3 h with different concentrations of perfluorohexane (PFH) emulsions, were monitored by microscopy, flow cytometry, and ultrasound. Effects of PFH loading on BMM adhesion molecule (PSGL-1, VLA-4, Mac-1, LFA-1) expression were analyzed by flow cytometry. Static adhesion of PFH loaded BMM to unstimulated and TNF-alpha stimulated b.End5 endothelial cells was assessed by microscopy. Incubation of BMM with PFH emulsions resulted in dose-dependent uptake and increased echogenicity (max. 17 dB). Flow cytometry analyses revealed no down-regulation related to PFH loading of BMM adhesion molecule expression. Endothelial adhesion remained functional, even after 24 h, although PFH loading dose-dependently attenuated static adhesion. PFH loaded BMM may potentially serve as ultrasound contrast agent for noninvasive detection of atherogenic hotspots in arteries
U2 - https://doi.org/10.1007/s11307-008-0146-3
DO - https://doi.org/10.1007/s11307-008-0146-3
M3 - Article
C2 - 18536974
SN - 1536-1632
VL - 10
SP - 264
EP - 270
JO - Molecular imaging and biology : MIB
JF - Molecular imaging and biology : MIB
IS - 5
ER -