Perfluorohexane-loaded macrophages as a novel ultrasound contrast agent: a feasibility study

Liselotte M. Kornmann, Daniëlle M. J. Curfs, Evelien Hermeling, Ingeborg van der Made, Menno P. J. de Winther, Robert S. Reneman, Koen D. Reesink, Arnold P. G. Hoeks

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

We investigated in vitro the potential of macrophages to act as targeted vehicle for ultrasound molecular imaging. Murine bone marrow-derived macrophages (BMM), incubated for 3 h with different concentrations of perfluorohexane (PFH) emulsions, were monitored by microscopy, flow cytometry, and ultrasound. Effects of PFH loading on BMM adhesion molecule (PSGL-1, VLA-4, Mac-1, LFA-1) expression were analyzed by flow cytometry. Static adhesion of PFH loaded BMM to unstimulated and TNF-alpha stimulated b.End5 endothelial cells was assessed by microscopy. Incubation of BMM with PFH emulsions resulted in dose-dependent uptake and increased echogenicity (max. 17 dB). Flow cytometry analyses revealed no down-regulation related to PFH loading of BMM adhesion molecule expression. Endothelial adhesion remained functional, even after 24 h, although PFH loading dose-dependently attenuated static adhesion. PFH loaded BMM may potentially serve as ultrasound contrast agent for noninvasive detection of atherogenic hotspots in arteries
Original languageEnglish
Pages (from-to)264-270
JournalMolecular imaging and biology : MIB
Volume10
Issue number5
DOIs
Publication statusPublished - 2008

Cite this