TY - JOUR
T1 - Pro-inflammatory functions of carp CXCL8-like and CXCb chemokines
AU - van der Aa, Lieke M.
AU - Chadzinska, Magdalena
AU - Golbach, Lieke A.
AU - Ribeiro, Carla M. S.
AU - Lidy Verburg-van Kemenade, B. M.
PY - 2012
Y1 - 2012
N2 - Numerous CXC chemokines have been identified in fish, however, their role in inflammation is not well established. Here. CXC chemokines of the CXCL8-like (CXCa_Ll and CXCL8_L2) and CXCL9/10/11-like (CXCb) subset were investigated in carp. Recombinant CXCa_L1, CXCL8_12 and CXCb all stimulated chemotaxis of macrophages and granulocytes in vitro. CXCb also attracted lymphocytes. Distinct effects on phagocyte activation were observed: the CXCL8-like chemokines increase respiratory burst activity, but not nitrite production. The three chemokines differentially induced a moderate increase in IL-1 II, CXCa_Ll and CXCL8_1.2 gene expression. Intracellular calcium mobilization in granulocytes upon CXCa_Ll stimulation implies signal transduction through G-protein coupled CXC receptors. Notably, upon intraperitoneal administration, carp CXCL8-like chemokines strongly induced in vivo leukocyte recruitment, including neutrophils and monocytes/macrophages, in contrast to CXCb, for which the number of recruited leukocytes was low. The results indicate functional homology for carp CXCL8-like and CXCb chemokines with mammalian CXCL8 and CXCL9-11, respectively. (C) 2011 Elsevier Ltd. All rights reserved
AB - Numerous CXC chemokines have been identified in fish, however, their role in inflammation is not well established. Here. CXC chemokines of the CXCL8-like (CXCa_Ll and CXCL8_L2) and CXCL9/10/11-like (CXCb) subset were investigated in carp. Recombinant CXCa_L1, CXCL8_12 and CXCb all stimulated chemotaxis of macrophages and granulocytes in vitro. CXCb also attracted lymphocytes. Distinct effects on phagocyte activation were observed: the CXCL8-like chemokines increase respiratory burst activity, but not nitrite production. The three chemokines differentially induced a moderate increase in IL-1 II, CXCa_Ll and CXCL8_1.2 gene expression. Intracellular calcium mobilization in granulocytes upon CXCa_Ll stimulation implies signal transduction through G-protein coupled CXC receptors. Notably, upon intraperitoneal administration, carp CXCL8-like chemokines strongly induced in vivo leukocyte recruitment, including neutrophils and monocytes/macrophages, in contrast to CXCb, for which the number of recruited leukocytes was low. The results indicate functional homology for carp CXCL8-like and CXCb chemokines with mammalian CXCL8 and CXCL9-11, respectively. (C) 2011 Elsevier Ltd. All rights reserved
U2 - https://doi.org/10.1016/j.dci.2011.11.011
DO - https://doi.org/10.1016/j.dci.2011.11.011
M3 - Article
C2 - 22182503
SN - 0145-305X
VL - 36
SP - 741
EP - 750
JO - Developmental and comparative immunology
JF - Developmental and comparative immunology
IS - 4
ER -