@article{407abdbd54694c4a957eb9a6b1fb1ed6,
title = "Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons",
abstract = "Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provide a protocol for the quantitative analysis of DCV fusion events in rodent neurons using pH-sensitive DCV fusion probes and custom-written analysis algorithms. This method can be used to study DCV fusion mechanisms and is easily adapted to investigate fusion principles of other secretory organelles. For complete details on the use and execution of this protocol, please refer to Persoon et al. (2019).",
keywords = "Cell culture, Cell membrane, Microscopy, Molecular/chemical probes, Neuroscience",
author = "Alessandro Moro and Hoogstraaten, {Rein I.} and Persoon, {Claudia M.} and Matthijs Verhage and Toonen, {Ruud F.}",
note = "Funding Information: The authors thank Robbert Zalm for cloning and producing lentiviral particles, Desiree Schut and Lisa Laan for astrocyte and neuronal cultures, Joke Wortel for animal breeding, Joost Hoetjes for genotyping, and members of the CNCR DCV project team for fruitful discussions. This work is supported by an ERC Advanced Grant ( 322966 ) of the European Union (to M.V.) Funding Information: The authors thank Robbert Zalm for cloning and producing lentiviral particles, Desiree Schut and Lisa Laan for astrocyte and neuronal cultures, Joke Wortel for animal breeding, Joost Hoetjes for genotyping, and members of the CNCR DCV project team for fruitful discussions. This work is supported by an ERC Advanced Grant (322966) of the European Union (to M.V.), R.I.H. and A.M. acquired data. R.I.H. A.M. C.M.P. M.V. and R.F.T. designed figures and wrote the protocol. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = mar,
day = "19",
doi = "https://doi.org/10.1016/j.xpro.2021.100325",
language = "English",
volume = "2",
pages = "1--18",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",
}
