Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons

Alessandro Moro, Rein I. Hoogstraaten, Claudia M. Persoon, Matthijs Verhage, Ruud F. Toonen

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5 Citations (Scopus)


Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provide a protocol for the quantitative analysis of DCV fusion events in rodent neurons using pH-sensitive DCV fusion probes and custom-written analysis algorithms. This method can be used to study DCV fusion mechanisms and is easily adapted to investigate fusion principles of other secretory organelles. For complete details on the use and execution of this protocol, please refer to Persoon et al. (2019).

Original languageEnglish
Article number100325
Pages (from-to)1-18
Number of pages18
JournalSTAR Protocols
Issue number1
Early online date3 Feb 2021
Publication statusPublished - 19 Mar 2021


  • Cell culture
  • Cell membrane
  • Microscopy
  • Molecular/chemical probes
  • Neuroscience

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