Restricted low-level human antibody responses against Epstein-Barr virus (EBV)-encoded latent membrane protein 1 in a subgroup of patients with EBV-associated diseases

P Meij; M B Vervoort; J Aarbiou; P van Dissel; A Brink; E Bloemena; C J Meijer; J M Middeldorp

doi:https://doi.org/10.1086/314704

Restricted low-level human antibody responses against Epstein-Barr virus (EBV)-encoded latent membrane protein 1 in a subgroup of patients with EBV-associated diseases

P Meij, M B Vervoort, J Aarbiou, P van Dissel, A Brink, E Bloemena, C J Meijer, J M Middeldorp

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Human antibody responses to latent membrane protein 1 (LMP1) in patients with Epstein-Barr virus (EBV)-related disease syndromes were analyzed in detail. Only by immunoblot analysis with purified recombinant LMP1 and by IFA on recombinant LMP1-expressing insect cells could human antibodies directed against LMP1 be detected. Low serum levels of LMP1-directed antibodies could be detected in 3 of 8 EBV-positive Hodgkin's disease patients, 3 of 40 nasopharyngeal carcinoma patients, 2 of 23 Burkitt's lymphoma patients, and 1 of 27 non-Burkitt's lymphoma patients. No LMP1-directed antibodies could be detected in healthy EBV carriers, infectious mononucleosis patients, or patients with chronic EBV disease. All sera contained significant levels of EBV antibodies directed against the immunodominant EBV proteins and peptides. From this study, it can be concluded that LMP1 is a protein with a very low immunogenicity for the humoral immune response in humans.

Original language	English
Pages (from-to)	1108-15
Number of pages	8
Journal	Journal of infectious diseases
Volume	179
Issue number	5
DOIs	https://doi.org/10.1086/314704
Publication status	Published - May 1999

Keywords

Amino Acid Sequence
Animals
Antibodies, Viral
Antigens, Viral
Burkitt Lymphoma
Enzyme-Linked Immunosorbent Assay
Epstein-Barr Virus Infections
Fluorescent Antibody Technique, Indirect
Herpesvirus 4, Human
Hodgkin Disease
Humans
Immunoblotting
Infectious Mononucleosis
Journal Article
Lymphoma
Mice
Molecular Sequence Data
Nasopharyngeal Neoplasms
Peptides
Recombinant Proteins
Research Support, Non-U.S. Gov't
Viral Matrix Proteins

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https://doi.org/10.1086/314704

Cite this

Meij, P., Vervoort, M. B., Aarbiou, J., van Dissel, P., Brink, A., Bloemena, E., Meijer, C. J., & Middeldorp, J. M. (1999). Restricted low-level human antibody responses against Epstein-Barr virus (EBV)-encoded latent membrane protein 1 in a subgroup of patients with EBV-associated diseases. Journal of infectious diseases, 179(5), 1108-15. https://doi.org/10.1086/314704

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title = "Restricted low-level human antibody responses against Epstein-Barr virus (EBV)-encoded latent membrane protein 1 in a subgroup of patients with EBV-associated diseases",

abstract = "Human antibody responses to latent membrane protein 1 (LMP1) in patients with Epstein-Barr virus (EBV)-related disease syndromes were analyzed in detail. Only by immunoblot analysis with purified recombinant LMP1 and by IFA on recombinant LMP1-expressing insect cells could human antibodies directed against LMP1 be detected. Low serum levels of LMP1-directed antibodies could be detected in 3 of 8 EBV-positive Hodgkin's disease patients, 3 of 40 nasopharyngeal carcinoma patients, 2 of 23 Burkitt's lymphoma patients, and 1 of 27 non-Burkitt's lymphoma patients. No LMP1-directed antibodies could be detected in healthy EBV carriers, infectious mononucleosis patients, or patients with chronic EBV disease. All sera contained significant levels of EBV antibodies directed against the immunodominant EBV proteins and peptides. From this study, it can be concluded that LMP1 is a protein with a very low immunogenicity for the humoral immune response in humans.",

keywords = "Amino Acid Sequence, Animals, Antibodies, Viral, Antigens, Viral, Burkitt Lymphoma, Enzyme-Linked Immunosorbent Assay, Epstein-Barr Virus Infections, Fluorescent Antibody Technique, Indirect, Herpesvirus 4, Human, Hodgkin Disease, Humans, Immunoblotting, Infectious Mononucleosis, Journal Article, Lymphoma, Mice, Molecular Sequence Data, Nasopharyngeal Neoplasms, Peptides, Recombinant Proteins, Research Support, Non-U.S. Gov't, Viral Matrix Proteins",

author = "P Meij and Vervoort, {M B} and J Aarbiou and {van Dissel}, P and A Brink and E Bloemena and Meijer, {C J} and Middeldorp, {J M}",

year = "1999",

month = may,

doi = "https://doi.org/10.1086/314704",

language = "English",

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Meij, P, Vervoort, MB, Aarbiou, J, van Dissel, P, Brink, A , Bloemena, E, Meijer, CJ & Middeldorp, JM 1999, 'Restricted low-level human antibody responses against Epstein-Barr virus (EBV)-encoded latent membrane protein 1 in a subgroup of patients with EBV-associated diseases', Journal of infectious diseases, vol. 179, no. 5, pp. 1108-15. https://doi.org/10.1086/314704

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AU - Meij, P

AU - Vervoort, M B

AU - Aarbiou, J

AU - van Dissel, P

AU - Brink, A

AU - Bloemena, E

AU - Meijer, C J

AU - Middeldorp, J M

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N2 - Human antibody responses to latent membrane protein 1 (LMP1) in patients with Epstein-Barr virus (EBV)-related disease syndromes were analyzed in detail. Only by immunoblot analysis with purified recombinant LMP1 and by IFA on recombinant LMP1-expressing insect cells could human antibodies directed against LMP1 be detected. Low serum levels of LMP1-directed antibodies could be detected in 3 of 8 EBV-positive Hodgkin's disease patients, 3 of 40 nasopharyngeal carcinoma patients, 2 of 23 Burkitt's lymphoma patients, and 1 of 27 non-Burkitt's lymphoma patients. No LMP1-directed antibodies could be detected in healthy EBV carriers, infectious mononucleosis patients, or patients with chronic EBV disease. All sera contained significant levels of EBV antibodies directed against the immunodominant EBV proteins and peptides. From this study, it can be concluded that LMP1 is a protein with a very low immunogenicity for the humoral immune response in humans.

AB - Human antibody responses to latent membrane protein 1 (LMP1) in patients with Epstein-Barr virus (EBV)-related disease syndromes were analyzed in detail. Only by immunoblot analysis with purified recombinant LMP1 and by IFA on recombinant LMP1-expressing insect cells could human antibodies directed against LMP1 be detected. Low serum levels of LMP1-directed antibodies could be detected in 3 of 8 EBV-positive Hodgkin's disease patients, 3 of 40 nasopharyngeal carcinoma patients, 2 of 23 Burkitt's lymphoma patients, and 1 of 27 non-Burkitt's lymphoma patients. No LMP1-directed antibodies could be detected in healthy EBV carriers, infectious mononucleosis patients, or patients with chronic EBV disease. All sera contained significant levels of EBV antibodies directed against the immunodominant EBV proteins and peptides. From this study, it can be concluded that LMP1 is a protein with a very low immunogenicity for the humoral immune response in humans.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Viral

KW - Antigens, Viral

KW - Burkitt Lymphoma

KW - Enzyme-Linked Immunosorbent Assay

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KW - Fluorescent Antibody Technique, Indirect

KW - Herpesvirus 4, Human

KW - Hodgkin Disease

KW - Humans

KW - Immunoblotting

KW - Infectious Mononucleosis

KW - Journal Article

KW - Lymphoma

KW - Mice

KW - Molecular Sequence Data

KW - Nasopharyngeal Neoplasms

KW - Peptides

KW - Recombinant Proteins

KW - Research Support, Non-U.S. Gov't

KW - Viral Matrix Proteins

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EP - 1115

JO - Journal of infectious diseases

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