Selective packaging of cellular miRNAs in HIV-1 particles

Nick C. T. Schopman; Thijs van Montfort; Marcel Willemsen; Stefanie A. Knoepfel; Georgios Pollakis; Antoine van Kampen; Rogier W. Sanders; Joost Haasnoot; Ben Berkhout

doi:https://doi.org/10.1016/j.virusres.2012.06.017

Selective packaging of cellular miRNAs in HIV-1 particles

Nick C. T. Schopman, Thijs van Montfort, Marcel Willemsen, Stefanie A. Knoepfel, Georgios Pollakis, Antoine van Kampen, Rogier W. Sanders, Joost Haasnoot, Ben Berkhout

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

Retroviral particles are known to package specific host cell components such as RNA molecules in addition to the two copies of the viral RNA genome. The highly sensitive SOLiD sequencing technology was used to determine the cellular miRNA content of human immunodeficiency virus type 1 (HIV-1) particles. We determined the relative concentration of cellular miRNAs in a T cell line and several primary cell subsets before and after HIV-1 infection, and compared those values to the miRNA content of virion particles. A small subset of the cellular miRNAs is dramatically concentrated in the virions up to 115 fold, suggesting a biological function in HIV-1 replication. (C) 2012 Elsevier B.V. All rights reserved

Original language	English
Pages (from-to)	438-447
Journal	Virus Research
Volume	169
Issue number	2
DOIs	https://doi.org/10.1016/j.virusres.2012.06.017
Publication status	Published - 2012

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https://doi.org/10.1016/j.virusres.2012.06.017

Cite this

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title = "Selective packaging of cellular miRNAs in HIV-1 particles",

abstract = "Retroviral particles are known to package specific host cell components such as RNA molecules in addition to the two copies of the viral RNA genome. The highly sensitive SOLiD sequencing technology was used to determine the cellular miRNA content of human immunodeficiency virus type 1 (HIV-1) particles. We determined the relative concentration of cellular miRNAs in a T cell line and several primary cell subsets before and after HIV-1 infection, and compared those values to the miRNA content of virion particles. A small subset of the cellular miRNAs is dramatically concentrated in the virions up to 115 fold, suggesting a biological function in HIV-1 replication. (C) 2012 Elsevier B.V. All rights reserved",

author = "Schopman, {Nick C. T.} and {van Montfort}, Thijs and Marcel Willemsen and Knoepfel, {Stefanie A.} and Georgios Pollakis and {van Kampen}, Antoine and Sanders, {Rogier W.} and Joost Haasnoot and Ben Berkhout",

year = "2012",

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journal = "Virus Research",

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T1 - Selective packaging of cellular miRNAs in HIV-1 particles

AU - Schopman, Nick C. T.

AU - van Montfort, Thijs

AU - Willemsen, Marcel

AU - Knoepfel, Stefanie A.

AU - Pollakis, Georgios

AU - van Kampen, Antoine

AU - Sanders, Rogier W.

AU - Haasnoot, Joost

AU - Berkhout, Ben

PY - 2012

Y1 - 2012

N2 - Retroviral particles are known to package specific host cell components such as RNA molecules in addition to the two copies of the viral RNA genome. The highly sensitive SOLiD sequencing technology was used to determine the cellular miRNA content of human immunodeficiency virus type 1 (HIV-1) particles. We determined the relative concentration of cellular miRNAs in a T cell line and several primary cell subsets before and after HIV-1 infection, and compared those values to the miRNA content of virion particles. A small subset of the cellular miRNAs is dramatically concentrated in the virions up to 115 fold, suggesting a biological function in HIV-1 replication. (C) 2012 Elsevier B.V. All rights reserved

AB - Retroviral particles are known to package specific host cell components such as RNA molecules in addition to the two copies of the viral RNA genome. The highly sensitive SOLiD sequencing technology was used to determine the cellular miRNA content of human immunodeficiency virus type 1 (HIV-1) particles. We determined the relative concentration of cellular miRNAs in a T cell line and several primary cell subsets before and after HIV-1 infection, and compared those values to the miRNA content of virion particles. A small subset of the cellular miRNAs is dramatically concentrated in the virions up to 115 fold, suggesting a biological function in HIV-1 replication. (C) 2012 Elsevier B.V. All rights reserved

U2 - https://doi.org/10.1016/j.virusres.2012.06.017

DO - https://doi.org/10.1016/j.virusres.2012.06.017

M3 - Article

C2 - 22728443

SN - 0168-1702

VL - 169

SP - 438

EP - 447

JO - Virus Research

JF - Virus Research

IS - 2

ER -