TY - JOUR
T1 - Sensitive ELISA for interleukin-6. Detection of IL-6 in biological fluids
T2 - synovial fluids and sera
AU - Helle, Maarten
AU - Boeije, Leonie
AU - de Groot, Els
AU - de Vos, Alex
AU - Aarden, Lucien
PY - 1991/4/8
Y1 - 1991/4/8
N2 - A monoclonal antibody and an affinity purified polyclonal antibody, both raised against recombinant human IL-6, have been employed in an ELISA procedure to quantitate human IL-6. Both antibodies were very potent in neutralizing the biological activity of recombinant as well as natural human IL-6. The monoclonal antibody was used as the capture antibody whilst the polyclonal antibody, in biotinylated form, was used as the detecting antibody in combination with a streptavidin horseradish peroxidase conjugate and a signal amplification system. The detection limit for natural as well as recombinant IL-6 was 1 pg/ml. A good correlation was found between the ELISA and the B9 biological assay when IL-6 was measured in crude culture supernatants, in synovial fluids of rheumatoid arthritis patients and in the sera of patients with diverse diseases. Immunoprecipitation of IL-6, produced by different cell types, such as monocytes, endothelial cells and smooth muscle cells or derived from biological fluids, such as the serum of a patient with septic shock or the synovial fluid of a rheumatoid arthritis patient, revealed in every case only molecules in the molecular weight range of 21,000-26,000.
AB - A monoclonal antibody and an affinity purified polyclonal antibody, both raised against recombinant human IL-6, have been employed in an ELISA procedure to quantitate human IL-6. Both antibodies were very potent in neutralizing the biological activity of recombinant as well as natural human IL-6. The monoclonal antibody was used as the capture antibody whilst the polyclonal antibody, in biotinylated form, was used as the detecting antibody in combination with a streptavidin horseradish peroxidase conjugate and a signal amplification system. The detection limit for natural as well as recombinant IL-6 was 1 pg/ml. A good correlation was found between the ELISA and the B9 biological assay when IL-6 was measured in crude culture supernatants, in synovial fluids of rheumatoid arthritis patients and in the sera of patients with diverse diseases. Immunoprecipitation of IL-6, produced by different cell types, such as monocytes, endothelial cells and smooth muscle cells or derived from biological fluids, such as the serum of a patient with septic shock or the synovial fluid of a rheumatoid arthritis patient, revealed in every case only molecules in the molecular weight range of 21,000-26,000.
KW - Amplification
KW - ELISA
KW - Interleukin-6
KW - Serum
UR - http://www.scopus.com/inward/record.url?scp=0025845509&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/0022-1759(91)90063-L
DO - https://doi.org/10.1016/0022-1759(91)90063-L
M3 - Article
C2 - 2019746
SN - 0022-1759
VL - 138
SP - 47
EP - 56
JO - Journal of immunological methods
JF - Journal of immunological methods
IS - 1
ER -