TY - JOUR
T1 - Sequence variation of the human immunodeficiency virus primer-binding site suggests the use of an alternative tRNA(Lys) molecule in reverse transcription
AU - Das, A. T.
AU - Klaver, B.
AU - Berkhout, B.
PY - 1997
Y1 - 1997
N2 - Retroviruses use a cellular tRNA molecule as primer for reverse transcription. The complementarity between the 3' end of this tRNA and a sequence near the 5' end of the viral RNA, the primer-binding site (PBS), allows the primer to anneal onto the viral RNA. During reverse transcription 18 nucleotides of the tRNA primer are copied into the viral cDNA, thereby regenerating the PBS sequence of the progeny. Thus, the PBS sequence reveals which primer was used. Human immunodeficiency viruses are known to replicate efficiently with tRNA(Lys3) as primer. Examination of the PBS sequence in natural and laboratory isolates indicates that a variant tRNA(Lys) is occasionally used as primer. This variant, for which the murine genomic sequence was described previously, was termed tRNA(Lys5) and differs from tRNA(Lys3) at five nucleotide positions. These results suggest that HIV uses both tRNA(Lys3) and tRNA(Lys5) molecules as primer, causing a switch of the PBS sequence
AB - Retroviruses use a cellular tRNA molecule as primer for reverse transcription. The complementarity between the 3' end of this tRNA and a sequence near the 5' end of the viral RNA, the primer-binding site (PBS), allows the primer to anneal onto the viral RNA. During reverse transcription 18 nucleotides of the tRNA primer are copied into the viral cDNA, thereby regenerating the PBS sequence of the progeny. Thus, the PBS sequence reveals which primer was used. Human immunodeficiency viruses are known to replicate efficiently with tRNA(Lys3) as primer. Examination of the PBS sequence in natural and laboratory isolates indicates that a variant tRNA(Lys) is occasionally used as primer. This variant, for which the murine genomic sequence was described previously, was termed tRNA(Lys5) and differs from tRNA(Lys3) at five nucleotide positions. These results suggest that HIV uses both tRNA(Lys3) and tRNA(Lys5) molecules as primer, causing a switch of the PBS sequence
U2 - https://doi.org/10.1099/0022-1317-78-4-837
DO - https://doi.org/10.1099/0022-1317-78-4-837
M3 - Article
C2 - 9129656
SN - 0022-1317
VL - 78
SP - 837
EP - 840
JO - Journal of general virology
JF - Journal of general virology
IS - 4
ER -