Small GTP-binding proteins in human endothelial cells

H. P. de Leeuw; P. M. Koster; J. Calafat; H. Janssen; A. J. van Zonneveld; J. A. van Mourik; J. Voorberg

doi:https://doi.org/10.1046/j.1365-2141.1998.00965.x

Small GTP-binding proteins in human endothelial cells

H. P. de Leeuw, P. M. Koster, J. Calafat, H. Janssen, A. J. van Zonneveld, J. A. van Mourik, J. Voorberg

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Abstract

Small GTP-binding proteins of the Ras superfamily control an extensive number of intracellular events by alternating between GDP- and GTP-bound conformation. The presence of members of this protein family was examined in human umbilical vein endothelial cells employing RT-PCR. Sequence analysis of 215 cDNA clones revealed the presence of a total of 28 different partial cDNAs encoding small GTP-binding proteins. Two sequences corresponded to novel isoforms of Rab2 and Rab9. In addition, human analogues of Rab4b, Rab7, Rab9, Rab14 and Rab15 were identified. Besides Rab proteins, members of other subfamilies were detected as well. As a first step towards elucidation of the function of the different small GTP-binding proteins identified we have isolated full length cDNA corresponding to Rab30 from a human endothelial cell cDNA library. In order to assess the subcellular localization of Rab30, we expressed epitope-tagged Rab30 cDNA in monkey kidney COS-1 cells. Immunoelectron-microscopy of transfected COS-1 cells indicated that Rab30 is associated with Golgi stacks

Original language	English
Pages (from-to)	15-19
Journal	British journal of haematology
Volume	103
Issue number	1
DOIs	https://doi.org/10.1046/j.1365-2141.1998.00965.x
Publication status	Published - 1998

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https://doi.org/10.1046/j.1365-2141.1998.00965.x

Cite this

@article{c653013bfb38446c91f64ccb4de7f054,

title = "Small GTP-binding proteins in human endothelial cells",

abstract = "Small GTP-binding proteins of the Ras superfamily control an extensive number of intracellular events by alternating between GDP- and GTP-bound conformation. The presence of members of this protein family was examined in human umbilical vein endothelial cells employing RT-PCR. Sequence analysis of 215 cDNA clones revealed the presence of a total of 28 different partial cDNAs encoding small GTP-binding proteins. Two sequences corresponded to novel isoforms of Rab2 and Rab9. In addition, human analogues of Rab4b, Rab7, Rab9, Rab14 and Rab15 were identified. Besides Rab proteins, members of other subfamilies were detected as well. As a first step towards elucidation of the function of the different small GTP-binding proteins identified we have isolated full length cDNA corresponding to Rab30 from a human endothelial cell cDNA library. In order to assess the subcellular localization of Rab30, we expressed epitope-tagged Rab30 cDNA in monkey kidney COS-1 cells. Immunoelectron-microscopy of transfected COS-1 cells indicated that Rab30 is associated with Golgi stacks",

author = "{de Leeuw}, {H. P.} and Koster, {P. M.} and J. Calafat and H. Janssen and {van Zonneveld}, {A. J.} and {van Mourik}, {J. A.} and J. Voorberg",

year = "1998",

doi = "https://doi.org/10.1046/j.1365-2141.1998.00965.x",

language = "English",

volume = "103",

pages = "15--19",

journal = "British journal of haematology",

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TY - JOUR

T1 - Small GTP-binding proteins in human endothelial cells

AU - de Leeuw, H. P.

AU - Koster, P. M.

AU - Calafat, J.

AU - Janssen, H.

AU - van Zonneveld, A. J.

AU - van Mourik, J. A.

AU - Voorberg, J.

PY - 1998

Y1 - 1998

N2 - Small GTP-binding proteins of the Ras superfamily control an extensive number of intracellular events by alternating between GDP- and GTP-bound conformation. The presence of members of this protein family was examined in human umbilical vein endothelial cells employing RT-PCR. Sequence analysis of 215 cDNA clones revealed the presence of a total of 28 different partial cDNAs encoding small GTP-binding proteins. Two sequences corresponded to novel isoforms of Rab2 and Rab9. In addition, human analogues of Rab4b, Rab7, Rab9, Rab14 and Rab15 were identified. Besides Rab proteins, members of other subfamilies were detected as well. As a first step towards elucidation of the function of the different small GTP-binding proteins identified we have isolated full length cDNA corresponding to Rab30 from a human endothelial cell cDNA library. In order to assess the subcellular localization of Rab30, we expressed epitope-tagged Rab30 cDNA in monkey kidney COS-1 cells. Immunoelectron-microscopy of transfected COS-1 cells indicated that Rab30 is associated with Golgi stacks

AB - Small GTP-binding proteins of the Ras superfamily control an extensive number of intracellular events by alternating between GDP- and GTP-bound conformation. The presence of members of this protein family was examined in human umbilical vein endothelial cells employing RT-PCR. Sequence analysis of 215 cDNA clones revealed the presence of a total of 28 different partial cDNAs encoding small GTP-binding proteins. Two sequences corresponded to novel isoforms of Rab2 and Rab9. In addition, human analogues of Rab4b, Rab7, Rab9, Rab14 and Rab15 were identified. Besides Rab proteins, members of other subfamilies were detected as well. As a first step towards elucidation of the function of the different small GTP-binding proteins identified we have isolated full length cDNA corresponding to Rab30 from a human endothelial cell cDNA library. In order to assess the subcellular localization of Rab30, we expressed epitope-tagged Rab30 cDNA in monkey kidney COS-1 cells. Immunoelectron-microscopy of transfected COS-1 cells indicated that Rab30 is associated with Golgi stacks

U2 - https://doi.org/10.1046/j.1365-2141.1998.00965.x

DO - https://doi.org/10.1046/j.1365-2141.1998.00965.x

M3 - Article

C2 - 9792283

SN - 0007-1048

VL - 103

SP - 15

EP - 19

JO - British journal of haematology

JF - British journal of haematology

IS - 1

ER -