TY - JOUR
T1 - Systemic and microcirculatory responses to progressive hemorrhage
AU - Dubin, Arnaldo
AU - Pozo, Mario Omar
AU - Ferrara, Gonzalo
AU - Murias, Gastón
AU - Martins, Enrique
AU - Canullán, Carlos
AU - Canales, Héctor Saul
AU - Kanoore Edul, Vanina Siham
AU - Estenssoro, Elisa
AU - Ince, Can
PY - 2009
Y1 - 2009
N2 - To compare systemic hemodynamics with microcirculatory changes at different vascular beds during progressive hemorrhage. University-based research laboratory. Twelve anesthetized, mechanically ventilated sheep. Sheep were randomly assigned to HEMORRHAGE or CONTROL group. In the HEMORRHAGE group (n = 8), three stepwise bleedings of 5 ml/kg at 30-min intervals were performed to add up 15 ml/kg. In the CONTROL group (n = 4), sheep had the same surgical preparation but were not bled. Progressive bleeding decreased cardiac output, and superior mesenteric artery blood flow, and systemic and intestinal oxygen transports from the first step of bleeding whereas systemic and intestinal oxygen consumption remained unchanged. Mean arterial blood pressure, arterial pH and base excess, and intramucosal-arterial PCO2 were only significantly modified in the last step of bleeding. Arterial lactate increased and sublingual, and intestinal serosal and mucosal capillary microvascular flow indexes and red blood cell velocities progressively decreased after the first step of bleeding (3.0 +/- A 0.1 vs. 2.3 +/- A 0.4, 3.2 +/- A 0.2 vs. 2.4 +/- A 0.6, 3.0 +/- A 0.0 vs. 2.0 +/- A 0.2, and 1,082 +/- A 29 vs. 977 +/- A 79, 1,042 +/- A 24 vs. 953 +/- A 60, 287 +/- A 65 vs. 262 +/- A 16 mu m/s; P <0.05 for all). Alterations in sublingual, intestinal microcirculation, and arterial lactate simultaneously arose from the first step of bleeding. The microcirculatory changes were identified either by semi-quantitative flow index or by quantitative red blood cell velocity measurements
AB - To compare systemic hemodynamics with microcirculatory changes at different vascular beds during progressive hemorrhage. University-based research laboratory. Twelve anesthetized, mechanically ventilated sheep. Sheep were randomly assigned to HEMORRHAGE or CONTROL group. In the HEMORRHAGE group (n = 8), three stepwise bleedings of 5 ml/kg at 30-min intervals were performed to add up 15 ml/kg. In the CONTROL group (n = 4), sheep had the same surgical preparation but were not bled. Progressive bleeding decreased cardiac output, and superior mesenteric artery blood flow, and systemic and intestinal oxygen transports from the first step of bleeding whereas systemic and intestinal oxygen consumption remained unchanged. Mean arterial blood pressure, arterial pH and base excess, and intramucosal-arterial PCO2 were only significantly modified in the last step of bleeding. Arterial lactate increased and sublingual, and intestinal serosal and mucosal capillary microvascular flow indexes and red blood cell velocities progressively decreased after the first step of bleeding (3.0 +/- A 0.1 vs. 2.3 +/- A 0.4, 3.2 +/- A 0.2 vs. 2.4 +/- A 0.6, 3.0 +/- A 0.0 vs. 2.0 +/- A 0.2, and 1,082 +/- A 29 vs. 977 +/- A 79, 1,042 +/- A 24 vs. 953 +/- A 60, 287 +/- A 65 vs. 262 +/- A 16 mu m/s; P <0.05 for all). Alterations in sublingual, intestinal microcirculation, and arterial lactate simultaneously arose from the first step of bleeding. The microcirculatory changes were identified either by semi-quantitative flow index or by quantitative red blood cell velocity measurements
U2 - https://doi.org/10.1007/s00134-008-1385-0
DO - https://doi.org/10.1007/s00134-008-1385-0
M3 - Article
C2 - 19127356
SN - 0342-4642
VL - 35
SP - 556
EP - 564
JO - Intensive care medicine
JF - Intensive care medicine
IS - 3
ER -