Target antigen of monoclonal reagent S5.7: comparison with T3 antigen

J. Borst; H. Spits; J. de Vries; S. Pessano; G. Rovera; C. Terhorst

doi:https://doi.org/10.1089/hyb.1983.2.265

Target antigen of monoclonal reagent S5.7: comparison with T3 antigen

J. Borst, H. Spits, J. de Vries, S. Pessano, G. Rovera, C. Terhorst

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

S5.7 recognizes a 20 kD cell surface protein which is present on T lymphocytes. S5.7 binds to a nonglycosylated protein, which can be labeled by cell-surface radioiodination and by a hydrophobic reagent [125I]-iodo-5-naphthyl-1-azide (INA). As the T-lymphocyte-specific T3 complex was found to contain a nonglycosylated 20 kD species, and since this 20 kD T3 form can be labeled preferentially by INA, a comparison between T3 and S5.7 was made. Isoelectric focusing experiments showed, however, that the two proteins are different. Moreover, the S5.7 monoclonal antibody does not block CML, is not mitogenic, reacts with immature cells of several hemopoietic lineages, and differs in that respect from anti-T3 monoclonal antibodies

Original language	English
Pages (from-to)	265-274
Journal	Hybridoma (2005)
Volume	2
Issue number	3
DOIs	https://doi.org/10.1089/hyb.1983.2.265
Publication status	Published - 1983

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https://doi.org/10.1089/hyb.1983.2.265

Cite this

@article{d5d51fa2a4364ebd8827070e27ffd94d,

title = "Target antigen of monoclonal reagent S5.7: comparison with T3 antigen",

abstract = "S5.7 recognizes a 20 kD cell surface protein which is present on T lymphocytes. S5.7 binds to a nonglycosylated protein, which can be labeled by cell-surface radioiodination and by a hydrophobic reagent [125I]-iodo-5-naphthyl-1-azide (INA). As the T-lymphocyte-specific T3 complex was found to contain a nonglycosylated 20 kD species, and since this 20 kD T3 form can be labeled preferentially by INA, a comparison between T3 and S5.7 was made. Isoelectric focusing experiments showed, however, that the two proteins are different. Moreover, the S5.7 monoclonal antibody does not block CML, is not mitogenic, reacts with immature cells of several hemopoietic lineages, and differs in that respect from anti-T3 monoclonal antibodies",

author = "J. Borst and H. Spits and {de Vries}, J. and S. Pessano and G. Rovera and C. Terhorst",

year = "1983",

doi = "https://doi.org/10.1089/hyb.1983.2.265",

language = "English",

volume = "2",

pages = "265--274",

journal = "Hybridoma (2005)",

issn = "1554-0014",

publisher = "Mary Ann Liebert Inc.",

number = "3",

}

TY - JOUR

T1 - Target antigen of monoclonal reagent S5.7: comparison with T3 antigen

AU - Borst, J.

AU - Spits, H.

AU - de Vries, J.

AU - Pessano, S.

AU - Rovera, G.

AU - Terhorst, C.

PY - 1983

Y1 - 1983

N2 - S5.7 recognizes a 20 kD cell surface protein which is present on T lymphocytes. S5.7 binds to a nonglycosylated protein, which can be labeled by cell-surface radioiodination and by a hydrophobic reagent [125I]-iodo-5-naphthyl-1-azide (INA). As the T-lymphocyte-specific T3 complex was found to contain a nonglycosylated 20 kD species, and since this 20 kD T3 form can be labeled preferentially by INA, a comparison between T3 and S5.7 was made. Isoelectric focusing experiments showed, however, that the two proteins are different. Moreover, the S5.7 monoclonal antibody does not block CML, is not mitogenic, reacts with immature cells of several hemopoietic lineages, and differs in that respect from anti-T3 monoclonal antibodies

AB - S5.7 recognizes a 20 kD cell surface protein which is present on T lymphocytes. S5.7 binds to a nonglycosylated protein, which can be labeled by cell-surface radioiodination and by a hydrophobic reagent [125I]-iodo-5-naphthyl-1-azide (INA). As the T-lymphocyte-specific T3 complex was found to contain a nonglycosylated 20 kD species, and since this 20 kD T3 form can be labeled preferentially by INA, a comparison between T3 and S5.7 was made. Isoelectric focusing experiments showed, however, that the two proteins are different. Moreover, the S5.7 monoclonal antibody does not block CML, is not mitogenic, reacts with immature cells of several hemopoietic lineages, and differs in that respect from anti-T3 monoclonal antibodies

U2 - https://doi.org/10.1089/hyb.1983.2.265

DO - https://doi.org/10.1089/hyb.1983.2.265

M3 - Article

C2 - 6236145

SN - 1554-0014

VL - 2

SP - 265

EP - 274

JO - Hybridoma (2005)

JF - Hybridoma (2005)

IS - 3

ER -