The effect of cytomegalovirus infection on T lymphocytes after allogeneic bone marrow transplantation

A M Würsch, J W Gratama, J M Middeldorp, C Nissen, A Gratwohl, B Speck, J Jansen, J D'Amaro, T H The, G C De Gast

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The influence of cytomegalovirus (CMV) infection on peripheral T lymphocyte repopulation was studied in 59 bone marrow transplant (BMT) recipients who received either cyclosporin A (CyA) or methotrexate (MTX) as prophylaxis for acute graft-versus-host disease. We used monoclonal antibodies and single- or double-marker immunofluorescence for the quantitation of T4+, T8+ and HNK1+ T cell subpopulations. CMV infection was serologically diagnosed by an enzyme-linked immunosorbent assay (ELISA), and by viral cultures and histological studies. Among the 52 patients who were evaluable for CMV infection, one had a primary infection and 24 had CMV reactivation/reinfection after BMT. In the latter patients, increases to supranormal levels were observed in T8+ T cells and HNK1+ T cells, both in patients on CyA and in patients on MTX. Double-marker immunofluorescence revealed that the two markers were largely expressed by the same cells, which therefore had the T8+ HNK1+ phenotype. In addition, the very small subset of T4+ HNK+ T cells was slightly, but consistently, increased in the patients with CMV reactivation/reinfection. CMV infection did not influence the numbers of T4+ HNK1- and T8+ HNK1- T cells. The long-lasting presence of large numbers of T8+ HNK1+ T cells in patients who had CMV reactivation/reinfection suggests a continuing interaction between the virus and the immune system of its host.

Original languageEnglish
Pages (from-to)278-87
Number of pages10
JournalClinical and experimental immunology
Issue number2
Publication statusPublished - Nov 1985


  • Adolescent
  • Adult
  • Antibodies, Viral
  • Bone Marrow Transplantation
  • Child
  • Cyclosporins
  • Cytomegalovirus
  • Cytomegalovirus Infections
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fluorescent Antibody Technique
  • Graft vs Host Disease
  • Humans
  • Immunoglobulin G
  • Immunoglobulin M
  • Journal Article
  • Leukemia
  • Leukocyte Count
  • Male
  • Methotrexate
  • Research Support, Non-U.S. Gov't
  • T-Lymphocytes

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