TY - JOUR
T1 - The influence of cytokines on the integrity of the blood-brain barrier in vitro
AU - De Vries, Helga E.
AU - Blom-Roosemalen, Margret C.M.
AU - Van Oosten, Marijke
AU - De Boer, Albert G.
AU - Van Berkel, Theo J.C.
AU - Breimer, Douwe D.
AU - Kuiper, Johan
PY - 1996
Y1 - 1996
N2 - The effects of the cytokines tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 Ω · cm2 on polycarbonate filters. Exposure of the RCEC to TNF-α, IL-1β and IL-6 induced a decline in the TEER, which could be completely abolished by 1 μM of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1β on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.
AB - The effects of the cytokines tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 Ω · cm2 on polycarbonate filters. Exposure of the RCEC to TNF-α, IL-1β and IL-6 induced a decline in the TEER, which could be completely abolished by 1 μM of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1β on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.
KW - Blood-brain barrier
KW - Cerebral endothelial cells
KW - Cytokines
KW - Inflammation
UR - http://www.scopus.com/inward/record.url?scp=0030034301&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/0165-5728(95)00148-4
DO - https://doi.org/10.1016/0165-5728(95)00148-4
M3 - Article
C2 - 8598388
SN - 0165-5728
VL - 64
SP - 37
EP - 43
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1
ER -