The influence of cytokines on the integrity of the blood-brain barrier in vitro

Helga E. De Vries, Margret C.M. Blom-Roosemalen, Marijke Van Oosten, Albert G. De Boer, Theo J.C. Van Berkel, Douwe D. Breimer, Johan Kuiper

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Abstract

The effects of the cytokines tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 Ω · cm2 on polycarbonate filters. Exposure of the RCEC to TNF-α, IL-1β and IL-6 induced a decline in the TEER, which could be completely abolished by 1 μM of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1β on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalJournal of Neuroimmunology
Volume64
Issue number1
DOIs
Publication statusPublished - 1996

Keywords

  • Blood-brain barrier
  • Cerebral endothelial cells
  • Cytokines
  • Inflammation

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