TY - JOUR
T1 - The N-5-glutamine S-adenosyl-L-methionine-dependent methyltransferase PrmC/HemK in Chlamydia trachomatis methylates class 1 release factors
AU - Pannekoek, Yvonne
AU - Heurgué-Hamard, Valérie
AU - Langerak, Ankie A. J.
AU - Speijer, Dave
AU - Buckingham, Richard H.
AU - van der Ende, Arie
PY - 2005
Y1 - 2005
N2 - The gene prmC, encoding the putative S-adenosyl-L-methionine (AdoMet) -dependent methyltransferase (MTase) of release factors (RFs) of the obligate intracellular pathogen Chlamydia trachomatis, was functionally analyzed. Chlamydial PrmC expression suppresses the growth defect of a prmC knockout strain of Escherichia coli K-12, suggesting an interaction of chlamydial PrmC with E. coli RFs in vivo. In vivo methylation assays carried out with recombinant PrmC and RFs of chlamydial origin demonstrated that PrmC methylates RFs within the tryptic fragment containing the universally conserved sequence motif Gly-Gly-Gln. This is consistent with the enzymatic properties of PrmC of E. coli origin. We conclude that C. trachomatis PrmC functions as an N-5-glutamine AdoMet-dependent MTase, involved in methylation of RFs
AB - The gene prmC, encoding the putative S-adenosyl-L-methionine (AdoMet) -dependent methyltransferase (MTase) of release factors (RFs) of the obligate intracellular pathogen Chlamydia trachomatis, was functionally analyzed. Chlamydial PrmC expression suppresses the growth defect of a prmC knockout strain of Escherichia coli K-12, suggesting an interaction of chlamydial PrmC with E. coli RFs in vivo. In vivo methylation assays carried out with recombinant PrmC and RFs of chlamydial origin demonstrated that PrmC methylates RFs within the tryptic fragment containing the universally conserved sequence motif Gly-Gly-Gln. This is consistent with the enzymatic properties of PrmC of E. coli origin. We conclude that C. trachomatis PrmC functions as an N-5-glutamine AdoMet-dependent MTase, involved in methylation of RFs
U2 - https://doi.org/10.1128/JB.187.2.507-511.2005
DO - https://doi.org/10.1128/JB.187.2.507-511.2005
M3 - Article
C2 - 15629922
SN - 0021-9193
VL - 187
SP - 507
EP - 511
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 2
ER -